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Dilution of Tris buffer solution 1

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Dilution of Tris buffer solution
1. How many fold do you dilute your 100 mM Tris buffer solution when preparing the 10 mM Tris 
buffer solution?
2. What is buffering capacity? Can water be used as a biological buffer?

 

Compare the myoglobin concentration found by OD280nm and OD410nm.

a. Are the concentrations found by two OD the same? 

b. Explain the reason(s) why are the concentrations the same / different base on the principles of Determination of myoglobin concentration by OD410 and Protein concentration determination by absorbance assay

 

Preparation of Microscopic Slides

1) During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?

2) Would 1 change of xylene for 20 minutes be superior to 2 changes of xylene for 10 minutes each in terms of dewaxing results? Explain. 

3) What is the typical composition of Harris Hematoxylin Solution? Which cellular organelle was more visible after immersing the intestinal cells in hematoxylin? 

4) What is the typical composition of Scott's tap water? Which cellular organelle was more visible after immersing the intestinal cells in Scott's tap water? 

5) What are the advantages of a medium-mounted slide over an un-mounted slide?

6) The section was put through a round of hydration after dewaxing and then subsequently a round of dehydration after eosin staining. Explain why such seemingly contradicting steps are essential.