Homework answers / question archive / Saddleback College BIO 12 PhysioEx 12: activity 4 Exercise 12: Serological Testing: Activity 4: Western Blotting Technique Lab Report Pre-lab Quiz Results 1)Which of the following tests is not correctly matched to what it tests for?   Gel electrophoresis is used to separate proteins on the basis of   Western blotting can be used to test for   In this activity you will use Western blotting to test for the presence of   Experiment Results Stop & Think Questions: What is present on the nitrocellulose strips?   How would you describe the binding site of the secondary antibody?   Experiment Data:     gp160 gp120 p55 p31 p24 HIV Test Result Patient A no no no no no Negative Patient B no no yes no yes Indeterminate Patient C yes yes yes no yes Positive Positive Control yes yes yes yes yes Positive Negative Control no no no no no Negative   Post-lab Quiz Results   The patient samples being tested contain   The primary antibodies in the Western blot   A key difference between the ELISA and the Western blot technique is that the   The antibodies in a positive patient sample bind to the     Review Sheet Results Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV

Saddleback College BIO 12 PhysioEx 12: activity 4 Exercise 12: Serological Testing: Activity 4: Western Blotting Technique Lab Report Pre-lab Quiz Results 1)Which of the following tests is not correctly matched to what it tests for?   Gel electrophoresis is used to separate proteins on the basis of   Western blotting can be used to test for   In this activity you will use Western blotting to test for the presence of   Experiment Results Stop & Think Questions: What is present on the nitrocellulose strips?   How would you describe the binding site of the secondary antibody?   Experiment Data:     gp160 gp120 p55 p31 p24 HIV Test Result Patient A no no no no no Negative Patient B no no yes no yes Indeterminate Patient C yes yes yes no yes Positive Positive Control yes yes yes yes yes Positive Negative Control no no no no no Negative   Post-lab Quiz Results   The patient samples being tested contain   The primary antibodies in the Western blot   A key difference between the ELISA and the Western blot technique is that the   The antibodies in a positive patient sample bind to the     Review Sheet Results Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV

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Review Sheet Results

1. Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV.  

2. Explain the procedure for a patient with an indeterminate HIV Western blot result.

3. Briefly describe how the nitrocellulose strips were prepared before reacting them with the patient samples.

4. Describe the importance of the washing steps in the procedure.