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University of Houston MICRO 3332 Quiz 7 1)The movement of an F factor from one cell to another requires a

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University of Houston

MICRO 3332

Quiz 7

1)The movement of an F factor from one cell to another requires

a.            A viral intermediate

 b. A sex pilus

c.             An Hfr cell

d.            DNA fragments in the environment

e.            A transposase

f.             An F- cell

g.            Two of the above

2.            The difference between generalized and specialized transduction is

a.            The size of the plasmids being moved

b.            The size of the phages being used

c.             That in generalized transduction, chromosomes are moved, whereas in specialized transduction, plasmids are moved

d.            That in generalized transduction, any DNA can be moved, but with specialized transduction, only certain DNA near the phage site can be moved

e.            There is no difference

3.                           is necessary if genes acquired by horizontal gene transfer are to become part of a bacterium’s genome

a.            Cell-to-cell contact

b.            Recombination

c.             Transposition

d.            A plasmid

e.            Hfr formation

4.            When    interacts with RNA polymerase, it increases the rate of transcription initiation of the lac operon

a.            Lacl protein

b.            Allolactose

c.             cAMP receptor protein

d.            Glucose

e.            Lactose

5.            What occurs when an inducer is added to a medium containing an organism with a metabolic pathway controlled by a repressor?

a.            The inducer combines with the repressor and activates the repressor

b.            The inducer combines with the repressor and inactivates the repressor

c.             The inducer combines with the substrate and blocks induction

d.            The inducer combines with the substrate and activates induction

e.            The inducer does not combine with, but functions as a chaperone molecule for, the enzyme-substrate complex

6.            The presence of tryptophan (trp) in E. coli

a.            Produces an inactive trp repressor

b.            Causes tryptophan to bind the trp operator and block transcription

 

c.             Causes tryptophan to act as an inducer of transcription of the trp operon

d.            Allows transcription of the trp structural genes

e.            None of the above

7.            High levels of transcription of the lactose operon require all of the following except

a.            The presence of lactose

b.            High levels of cAMP (cyclic AMP)

c.             The presence of glucose

d.            Inactive repressor

e.            The formation of allolactose

f.             Two of the above are not needed for high levels of transcription of the lactose operon

8.            The control of the lactose and tryptophan operons, respectively, via the action of repressor proteins is an example of

a.            Transcriptional control

b.            Post-transcriptional control

c.             Post-translational control

d.            None of the above

9.            A segment of DNA that moves from one part of a chromosome to a different location in the same chromosome

a.            Is a process that occurs via transformation

b.            Is how a transposon functions

c.             Is the process of specialized transduction

d.            Leads to formation of an F+ cell

10.          The function of the trp operon gene products is to                          tryptophan. The function of the lac operon gene products is to         lactose

a.            Catabolize; synthesize

b.            Synthesize; synthesize

c.             Synthesize; catabolize

d.            Catabolize; catabolize

e.            Chaperone; recombine

11.          Assume the two E. coli strains shown below are allowed to conjugate. Hfr strain: pro+, arg+, his+, lys+, met+, amp-sensitive

F- strain: pro-, arg-, his-, lys-, met-, amp-resistant

What supplements would you add to glucose minimal salts agar to select for recombinant F- cells that are lys+ and arg+?

a.            Lysine, arginine

b.            Proline, histidine, methionine

c.             Ampicillin, lysine, arginine

d.            Ampicillin, proline, histidine, lysine

e.            Ampicillin, proline, histidine, methionine

12.          Transposition requires

a.            Cell to cell contact

b.            A viral intermediate

c.             A sex pilus

 

d.            An Hfr cell

e.            DNA fragments in the environment

f.             A transposase

g.            Two of the above