University of South Florida, St. Petersburg

PCB 3713L

Exercise 8: Chemical and Physical Processes of Digestion: Activity 3: Assessing Pepsin Digestion of Protein Lab Report

Pre-lab Quiz Results

1)Where in the body does protein digestion begin?

2. The substrate for pepsin is

3. In this activity the substrate you will be using to detect protein digestion is

4. Negative results with the negative controls

5. A spectrophometer measures

Experiment Results Predict Question:

Predict Question1: At which pH do you think pepsin will have the highest activity?

Stop & Think Questions:

2. Which of the following tubes are negative control tubes?

3. An optical density greater than zero indicates that

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. Pepsin would be most active

2. Which two tubes validated the results of the experiment?  

3. With more enzyme activity the optical density

4. If pepsin were digesting an actual protein substrate, the product would be