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Homework answers / question archive / Medical Microbiology & Techniques Worksheet for Labs #7-8 Begin in Lab 7

Medical Microbiology & Techniques Worksheet for Labs #7-8 Begin in Lab 7

Biology

Medical Microbiology & Techniques

Worksheet for Labs #7-8

Begin in Lab 7. This must be completed before conducting tests to identify your isolated unknown.

 

  1. Using Bergey’s Manual or similar sources complete the following chart with the expected results for each of the tests. The chart only needs to be completed to the extent that you can positively identify each of the possible unknowns (i.e., you do not need every single result). Please see the Guide to Dichotomous Keys (in the Lab 6 manual) before you begin this exercise.

 

Test

C. freundii

E. aerogenes

E. coli

K. pneumoniae

M. morganii

P. mirabilis

P. vulgaris

P. aeruginosa

S. gallinarum

S. marcescens

Growth @ RT

Slow to average growth

Average to good growth

Average growth

Average to good growth

Average to good growth

Good growth (optimum)

Good growth (optimum)

Average to good gowth

Average to good growth

 

Good growth (near optimum)

Growth @ 37°C

 

Good growth (optimum)

 

Good growth (optimum)

 

Good growth (optimum)

 

Good growth (optimum)

 

Good growth (optimum)

 

Average to good growth

Average to good growth

 

Good growth (optimum)

 

Good growth (optimum)

 

 

Average to good growth

TSA colony appear.

Cream white colonies, flat

Cream white colonies, flat

Cream white colonies, flat to elevated

Cream white, large mucoid colonies

White to cream, opaque colonies

Swarming white colonies

Swarming white colonies

Swarming white with green pigment colonies

Cream white colonies, flat

Red colonies

MAC

 

 

Colonies are light pink

Large, pink, mucoid

Large, red, surrounded by turbid zone

Large, pink, mucoid

Swarming opaque colonies

Swarming opaque colonies

Swarming opaque colonies

Swarming opaque colonies

Very small, opaque, isolated colonies

Colonies may have red pigment

EMB

 

 

Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light

Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light

Blue-black colonies with green metallic sheen

Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light

Colorless colonies

Colorless colonies

Colorless colonies

Colorless colonies

No growth to very small, colorless colonies

Colorless colonies

Oxidase

 

 

 

_

_

+

_

_

_

_

 

+

_

_

O-F test (un-sealed)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

Yellow (oxidative)

 

Yellow (oxidative)

 

O-F test (sealed)

 

Yellow (fermentative)

Yellow (fermentative)

Yellow (fermentative)

Yellow (fermentative)

Yellow (fermentative)

Yellow (fermentative)

Yellow (fermentative)

Green -

Yellow (fermentative)

Yellow (fermentative)

Glucose fermn.

 

 

+gas

 

 

 

+acid

+acid

+acid

+acid

+acid

+acid

No gas

+acid

 

No gas

 

 

Lactose fermn.

Acid +

Acid +

Acid with gas +

Acid +

_

_

_

_

+

 

_

 

Citrate

+

 

 

+

 

_

 

 

 

_

 

+

+

 

_

 

+

+

 

 

+

 

 

 

Test

C. freundii

E. aerogenes

E. coli

K. pneumoniae

M. morganii

P. mirabilis

P. vulgaris

P. aeruginosa

S. gallinarum

S. marcescens

MR

 

+

_

+

_

 

+

+

+

_

+

_

 

VP

 

_

+

_

+

_

_

 

 

_

_

+

 

+

 

Indole

_

 

+

_

+

_

 

+

_

_

_

TSI

 

 

 

H2S+

Lysine+

H2S-

 

H2S-

Lysine+

H2S-

 

 

 

H2S-

Sucrose-

 

 

 

 

Sucrose fermented

 

H2S+

Sucrose+

 

H2S-

H2S-

 

 

H2S-

 

 

Urease

_

 

_

_

+

+

+

+

_

+

_

 

 

 

Motility

+

 

+

+

_

+

+

+

+

_

 

+

 

 

 

 

 

  1. Prepare two dichotomous keys/flow-charts for the determination of your unknowns. Try your best to use unique tests in each flow-chart, or find alternate tests in “major” branch points in your tree. (Keys/charts can be printed/drawn on separate pages and attached.)

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