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Homework answers / question archive / Medical Microbiology & Techniques Worksheet for Labs #7-8 Begin in Lab 7
Medical Microbiology & Techniques
Worksheet for Labs #7-8
Begin in Lab 7. This must be completed before conducting tests to identify your isolated unknown.
Test |
C. freundii |
E. aerogenes |
E. coli |
K. pneumoniae |
M. morganii |
P. mirabilis |
P. vulgaris |
P. aeruginosa |
S. gallinarum |
S. marcescens |
Growth @ RT |
Slow to average growth |
Average to good growth |
Average growth |
Average to good growth |
Average to good growth |
Good growth (optimum) |
Good growth (optimum) |
Average to good gowth |
Average to good growth |
Good growth (near optimum) |
Growth @ 37°C |
Good growth (optimum)
|
Good growth (optimum)
|
Good growth (optimum)
|
Good growth (optimum)
|
Good growth (optimum)
|
Average to good growth |
Average to good growth
|
Good growth (optimum)
|
Good growth (optimum)
|
Average to good growth |
TSA colony appear. |
Cream white colonies, flat |
Cream white colonies, flat |
Cream white colonies, flat to elevated |
Cream white, large mucoid colonies |
White to cream, opaque colonies |
Swarming white colonies |
Swarming white colonies |
Swarming white with green pigment colonies |
Cream white colonies, flat |
Red colonies |
MAC
|
Colonies are light pink |
Large, pink, mucoid |
Large, red, surrounded by turbid zone |
Large, pink, mucoid |
Swarming opaque colonies |
Swarming opaque colonies |
Swarming opaque colonies |
Swarming opaque colonies |
Very small, opaque, isolated colonies |
Colonies may have red pigment |
EMB
|
Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light |
Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light |
Blue-black colonies with green metallic sheen |
Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light |
Colorless colonies |
Colorless colonies |
Colorless colonies |
Colorless colonies |
No growth to very small, colorless colonies |
Colorless colonies |
Oxidase
|
_ |
_ |
+ |
_ |
_ |
_ |
_ |
+ |
_ |
_ |
O-F test (un-sealed) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative) |
Yellow (oxidative)
|
O-F test (sealed)
|
Yellow (fermentative) |
Yellow (fermentative) |
Yellow (fermentative) |
Yellow (fermentative) |
Yellow (fermentative) |
Yellow (fermentative) |
Yellow (fermentative) |
Green - |
Yellow (fermentative) |
Yellow (fermentative) |
Glucose fermn. |
+gas
|
+acid |
+acid |
+acid |
+acid |
+acid |
+acid |
No gas |
+acid |
No gas
|
Lactose fermn. |
Acid + |
Acid + |
Acid with gas + |
Acid + |
_ |
_ |
_ |
_ |
+ |
_
|
Citrate |
+
|
+ |
_
|
_ |
+ |
+
|
_
|
+ |
+ |
+ |
Test |
C. freundii |
E. aerogenes |
E. coli |
K. pneumoniae |
M. morganii |
P. mirabilis |
P. vulgaris |
P. aeruginosa |
S. gallinarum |
S. marcescens |
MR
|
+ |
_ |
+ |
_
|
+ |
+ |
+ |
_ |
+ |
_
|
VP |
_ |
+ |
_ |
+ |
_ |
_
|
_ |
_ |
+ |
+
|
Indole |
_
|
+ |
_ |
+ |
_ |
|
+ |
_ |
_ |
_ |
TSI |
H2S+ |
Lysine+ H2S- |
H2S- |
Lysine+ H2S- |
H2S- Sucrose- |
Sucrose fermented
|
H2S+ Sucrose+ |
H2S- |
H2S- |
H2S-
|
Urease |
_
|
_ |
_ |
+ |
+ |
+ |
+ |
_ |
+ |
_
|
Motility |
+
|
+ |
+ |
_ |
+ |
+ |
+ |
+ |
_ |
+
|
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