Medical Microbiology & Techniques Worksheet for Labs #7-8 Begin in Lab 7. This must be completed before conducting tests to identify your isolated unknown. Using Bergey’s Manual or similar sources complete the following chart with the expected results for each of the tests. The chart only needs to be completed to the extent that you can positively identify each of the possible unknowns (i.e., you do not need every single result). Please see the Guide to Dichotomous Keys (in the Lab 6 manual) before you begin this exercise. Test C. freundii E. aerogenes E. coli K. pneumoniae M. morganii P. mirabilis P. vulgaris P. aeruginosa S. gallinarum S. marcescens Growth @ RT Slow to average growth Average to good growth Average growth Average to good growth Average to good growth Good growth (optimum) Good growth (optimum) Average to good gowth Average to good growth Good growth (near optimum) Growth @ 37°C Good growth (optimum) Good growth (optimum) Good growth (optimum) Good growth (optimum)

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Medical Microbiology & Techniques Worksheet for Labs #7-8 Begin in Lab 7

Biology Mar 18, 2021

Medical Microbiology & Techniques

Worksheet for Labs #7-8

Begin in Lab 7. This must be completed before conducting tests to identify your isolated unknown.

Using Bergey’s Manual or similar sources complete the following chart with the expected results for each of the tests. The chart only needs to be completed to the extent that you can positively identify each of the possible unknowns (i.e., you do not need every single result). Please see the Guide to Dichotomous Keys (in the Lab 6 manual) before you begin this exercise.

Test	C. freundii	E. aerogenes	E. coli	K. pneumoniae	M. morganii	P. mirabilis	P. vulgaris	P. aeruginosa	S. gallinarum	S. marcescens
Growth @ RT	Slow to average growth	Average to good growth	Average growth	Average to good growth	Average to good growth	Good growth (optimum)	Good growth (optimum)	Average to good gowth	Average to good growth	Good growth (near optimum)
Growth @ 37°C	Good growth (optimum)	Good growth (optimum)	Good growth (optimum)	Good growth (optimum)	Good growth (optimum)	Average to good growth	Average to good growth	Good growth (optimum)	Good growth (optimum)	Average to good growth
TSA colony appear.	Cream white colonies, flat	Cream white colonies, flat	Cream white colonies, flat to elevated	Cream white, large mucoid colonies	White to cream, opaque colonies	Swarming white colonies	Swarming white colonies	Swarming white with green pigment colonies	Cream white colonies, flat	Red colonies
MAC	Colonies are light pink	Large, pink, mucoid	Large, red, surrounded by turbid zone	Large, pink, mucoid	Swarming opaque colonies	Swarming opaque colonies	Swarming opaque colonies	^{Swarming opaque colonies}	Very small, opaque, isolated colonies	Colonies may have red pigment
EMB	Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light	Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light	Blue-black colonies with green metallic sheen	Colonies are larger than those of E.coli, mucoid, confluent, gray-brown centre in transmitted light	Colorless colonies	Colorless colonies	Colorless colonies	Colorless colonies	No growth to very small, colorless colonies	Colorless colonies
Oxidase	_	_	+	_	_	_	_	+	_	_
O-F test (un-sealed)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)	Yellow (oxidative)
O-F test (sealed)	Yellow (fermentative)	Yellow (fermentative)	Yellow (fermentative)	Yellow (fermentative)	Yellow (fermentative)	Yellow (fermentative)	Yellow (fermentative)	Green -	Yellow (fermentative)	Yellow (fermentative)
Glucose fermn.	+gas	+acid	+acid	+acid	+acid	+acid	+acid	No gas	+acid	No gas
Lactose fermn.	Acid +	Acid +	Acid with gas +	Acid +	_	_	_	_	+	_
Citrate	+	+	_	_	+	+	_	+	+	+

Test	C. freundii	E. aerogenes	E. coli	K. pneumoniae	M. morganii	P. mirabilis	P. vulgaris	P. aeruginosa	S. gallinarum	S. marcescens
MR	+	_	+	_	+	+	+	_	+	_
VP	_	+	_	+	_	_	_	_	+	+
Indole	_	+	_	+	_		+	_	_	_
TSI	H₂S+	Lysine+ H₂S-	H₂S-	Lysine+ H₂S-	H₂S- Sucrose-	Sucrose fermented	H₂S+ Sucrose+	H₂S-	H₂S-	H₂S-
Urease	_	_	_	+	+	+	+	_	+	_
Motility	+	+	+	_	+	+	+	+	_	+

Prepare two dichotomous keys/flow-charts for the determination of your unknowns. Try your best to use unique tests in each flow-chart, or find alternate tests in “major” branch points in your tree. (Keys/charts can be printed/drawn on separate pages and attached.)

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