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When phage λ is grown on a Tn10-containing E

Biology

When phage λ is grown on a Tn10-containing E. coli host, rare phage that transduce tetracycline-resistance can be isolated. You isolate DNA from λ and from λ-tet and cut each with EcoRI and BamHI and see the following sized fragments on an agarose gel:

Tn10 is 9kb long, with one EcoRI site 3.5kb from its left end (and there are no BamHI sites).

Construct a restriction map of λ showing the positions of the EcoRI and BamHI sites and the position and orientation of the Tn10 insertion in λ-tet.

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