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1.) Why are antibiotics used in engineering plasmid vectors? 

2.) When designing a bacterial plasmid vector to be utilized in an in-Vivo experiment using eukaryotic cells, should you incorporate antibiotics into the plasmid vector? Why or why not? 

3.) Benefits and negatives of using Luciferase and GFP when determining the location of the products of a gene of interest within eukaryotic cells. Which would be more efficient and effective?