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Saddleback College BIO 12 Exercise 12: Serological Testing: Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Lab Report Pre-lab Quiz Results 1)For home pregnancy tests Which of the following is true of the indirect ELISA? What does the antigen bind to on the antibody? Which of the following is true of the direct ELISA? Experiment Results Stop & Think Questions: What must occur in order for an HIV-infected individual to test positive with the indirect ELISA test? What is the key substance found in the developing buffer? Experiment Data: Sample Optical Density HIV Test Result Positive Control 1
Saddleback College
BIO 12
Exercise 12: Serological Testing: Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Lab Report Pre-lab Quiz Results
1)For home pregnancy tests
- Which of the following is true of the indirect ELISA?
- What does the antigen bind to on the antibody?
- Which of the following is true of the direct ELISA?
Experiment Results Stop & Think Questions:
- What must occur in order for an HIV-infected individual to test positive with the indirect ELISA test?
- What is the key substance found in the developing buffer?
Experiment Data:
|
Sample |
Optical Density |
HIV Test Result |
|
Positive Control |
1.624 |
Positive |
|
Negative Control |
0.154 |
Negative |
|
Patient A |
0.054 |
Negative |
|
Patient B |
0.432 |
Negative |
|
Patient C |
1.990 |
Positive |
Post-lab Quiz Results
- Antibodies are made of
- How would you describe the binding site of the secondary antibody?
- A positive result for HIV in the indirect ELISA performed contains
- Which patient needs to be retested?
Review Sheet Results
-
- Describe how the direct and indirect ELISA are different.
-
- Discuss why a patient might test indeterminate.
-
- How would your results have been affected if your negative control had given an indeterminate result?
-
- Briefly describe the basic structure of antibodies.
Expert Solution
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