Texas Southern University - BIOL 347

Chapter 19

Applications of Immune Responses

Matching Questions

1)Which of the following best matches the description:

1. Flow cytometry            Western blot  
2. color development      fluorescent activated cell sorter    
3. nylon filter      indirect fluorescent antibody test  
4. fluorescent microscope           ELISA    
5. agarose gel                  Ouchterlony procedure    

2. Which of the following best matches the description

1. child recovered from chickenpox            naturally acquired passive  
2. immunization with measles vaccine      naturally acquired active         artificially acquired
3. gamma globulin injections     passive  
4. immunity acquired by a baby from mother's

milk          artificially acquired active  

True / False Questions

1. Active immunity develops only after a natural infection and not after vaccination.

2. Attenuated agents often give rise to a long-lasting immunity.

3. Inactivated vaccines typically require booster shots.

4. Recombinant vaccines and inactivated vaccines typically require several shots to be effective.

5. Alum is an adjuvant.

6. An adjuvant has been developed from Lipid A

7. Peptide vaccines have proven to be cheap and very immunogenic.

8. The effectiveness of DNA vaccines stems from the effective production of antibodies against the naked DNA molecule.

9. DNA vaccines are dangerous due to the possibility of the DNA causing reversion in the inactivated pathogen.

10. The risk of serious illness from measles is 500 times greater than from vaccination against the disease.

11. Agglutination reactions utilize particles rather than molecules.

12. A monoclonal antibody is typically IgE and recognizes several epitopes.

Essay Questions

13. What was critical to the elimination of smallpox? Could the success in eliminating smallpox be applied to other diseases? Give an example of another disease that may be eliminated due to vaccination.

14. What key characteristic of B cells makes it possible to produce monoclonal antibodies? What else was necessary to make the production of monoclonal antibodies practical?