Texas Southern University - BIOL 347

Chapter 09

Biotechnology and Recombinant DNA

Matching Questions

1)Which of the following best matches the description:

1. Molecular scissors       
2. Plasmid            
3. A technique used to identify fragments of DNA on a

nylon membrane              

4. A small piece of tagged DNA      recombinant DNA  
5. Two different pieces of DNA linked together              

True / False Questions

2. Agarose gel electrophoresis may be considered as a partial purification technique.
3. Most eukaryotic genes are cloned directly into the vector for expression in prokaryotes.

4. PCR is useful for amplifying a particular section of DNA.

5. PCR typically results in the generation of fragments of all sizes.

6. Vectors must have at least one restriction enzyme recognition site.

7. A very common vector is a plasmid.

8. When using lacZ' containing vectors, colonies containing intact vector turn blue.

9. DNA probes are used to find regions of complementary DNA.

10. FISH uses labeled probes to detect specific whole cells.
11. A DNA microarray contains oligonucleotides that contain a label.

Essay Questions

12. What is the problem with putting a eukaryotic gene into a prokaryote?

13. Why is a DNA fragment generated by PCR of essentially one particular size?