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Given the SNP genotyping information about the following individual: Individual 1: Aa Bb CC Dd Individual 2: aa bb CC DD Individual 3: Aa Bb CC DD Individual 4: aa Bb CC dd Individual 5: aa Bb CC Dd Use the Clark's algorithm to resolve the haplotypes for each individual

Biology Oct 07, 2020

Given the SNP genotyping information about the following individual:

Individual 1: Aa Bb CC Dd
Individual 2: aa bb CC DD
Individual 3: Aa Bb CC DD
Individual 4: aa Bb CC dd
Individual 5: aa Bb CC Dd

Use the Clark's algorithm to resolve the haplotypes for each individual. E.g. Individual 1 = (text field aBCd) + (text field AbCD)

Expert Solution

Clark's algorithm begins by finding all homozygotes and single-site heterozygotes
and resolving the resulting known haplotypes.

Homozygotes

Individual 2: (abCD) + (abCD)

Single-site Heterozygotes

Individual 4: (aBCd) + (abCd)

Then, for each known haplotype, look at all the remaining unresolved sequences
and ask whether the known haplotype can be made from some combination of the
ambiguous sites. Each time such a haplotype is found, recover the complement of the
haplotype as another potential haplotype. This chain of inference
continues until all haplotypes have been recovered, or until no more new haplotypes
can be found.

Individual 1: (abCD) from individual 2 + (ABCd) complement
(aBCd) from individual 4 + (AbCD) complement
(abCd) from individual 4 + (ABCD) complement

Individual 3: (abCD) from individual 2 + (ABCD) complement
(AbCD) from individual 1 + (aBCD) complement

Individual 5: (abCD) from individual 2 + (aBCD) complement
(abCd) from individual 4 + (aBCD) complement

Going back to check for the above haplotypes

Individual 1: (aBCD) from individual 3 + (AbCd) complement

All haplotypes have been resolved:-

Individual 1: (abCD) + (ABCd)
(aBCd) + (AbCD)
(abCd) + (ABCD)
(AbCd) + (aBCD)

Individual 2: (abCD) + (abCD)

Individual 3: (abCD) + (ABCD)
(aBCD) + (AbCD)

Individual 4: (aBCd) + (abCd)

Individual 5: (aBCD) + (abCd);
(aBCd) + (abCD)

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