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(1) Why should long stretches of one nucleotide like AAAAAAA OR nucleotide repeats like ATATATATAT avoided? Why should both primers be designed with similar annealing temperatures, or even better, the SAME annealing temperature?
(1) Why should long stretches of one nucleotide like AAAAAAA OR nucleotide repeats like ATATATATAT avoided?
Why should both primers be designed with similar annealing temperatures, or even better, the SAME annealing temperature?
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