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(1) Why should long stretches of one nucleotide like AAAAAAA OR nucleotide repeats like ATATATATAT avoided?     Why should both primers be designed with similar annealing temperatures, or even better, the SAME annealing temperature?

Biology Jan 28, 2023

(1) Why should long stretches of one nucleotide like AAAAAAA OR nucleotide repeats like ATATATATAT avoided?

 

 

Why should both primers be designed with similar annealing temperatures, or even better, the SAME annealing temperature?

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