Homework answers / question archive / Here’s what you’ll need… Just about everyone has heard of DNA, but do you know that it is possible to isolate DNA with things you probably have in your kitchen? Dry split peas, table salt, water, dishwashing detergent, meat tenderizer, 91% isopropyl rubbing alcohol, a blender and a strainer Ready, set, let’s isolate some DNA… Add the following to a blender -Approximately half a cup of dry split peas -A large pinch of table salt (about 1/8 teaspoon) -About 1 cup of COLD water Pulse the blender at high speed for about 10 seconds; do not over blend or the DNA will be fragmented Pour through a strainer into another container Add about 2 tablespoons of dishwashing detergent; swirl gently to mix…DO NOT STIR

Here’s what you’ll need… Just about everyone has heard of DNA, but do you know that it is possible to isolate DNA with things you probably have in your kitchen? Dry split peas, table salt, water, dishwashing detergent, meat tenderizer, 91% isopropyl rubbing alcohol, a blender and a strainer Ready, set, let’s isolate some DNA… Add the following to a blender -Approximately half a cup of dry split peas -A large pinch of table salt (about 1/8 teaspoon) -About 1 cup of COLD water Pulse the blender at high speed for about 10 seconds; do not over blend or the DNA will be fragmented Pour through a strainer into another container Add about 2 tablespoons of dishwashing detergent; swirl gently to mix…DO NOT STIR

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Here’s what you’ll need… Just about everyone has heard of DNA, but do you know that it is possible to isolate DNA with things you probably have in your kitchen? Dry split peas, table salt, water, dishwashing detergent, meat tenderizer, 91% isopropyl rubbing alcohol, a blender and a strainer Ready, set, let’s isolate some DNA… Add the following to a blender -Approximately half a cup of dry split peas -A large pinch of table salt (about 1/8 teaspoon) -About 1 cup of COLD water Pulse the blender at high speed for about 10 seconds; do not over blend or the DNA will be fragmented Pour through a strainer into another container Add about 2 tablespoons of dishwashing detergent; swirl gently to mix…DO NOT STIR. Let sit for 5-10 minutes. Pour a small amount (~ 5 mls) into a small tube Now here’s where you come in… -Add a pinch of meat tenderizer to the tube and mix the tube gently -Tilt the tube to the side and slowly add a pipette full of isopropyl rubbing alcohol…add the alcohol slowly down the side of the tube so that it forms a layer on top of the original mixture…DO NOT ADD THE ALCOHOL ALL AT ONCE -In a few minutes you should notice a white substance floating above the original green material -This white stringy fiber is the DNA! Use a hook to gently loop the DNA; if it is difficult to remove, carefully press it to the side and pull it up the side of the container. Place the DNA in a small container filled with isopropyl alcohol and cap tightly! DNA can be isolated from many fruits and vegetables...give it a try at home! ©2014 Kathryn M Nette Exercise15: DNA, Genes, Chromosomes and Cancer Skill Check Worksheet NAME:_____________________________ Section:____________________________ Activity 1: Extraction of DNA 1) Explain why you think each of the following steps of the procedures was necessary to the extraction process: a) Blending the mixture of peas, salt and water in the blender b) Adding the detergent to the mixture c) Adding the meat tenderizer 2) How might you go about proving that the material that was extracted is really DNA? 3) What other sources could be used to extract DNA? Activity 2: DNA, Genes, Chromosomes and Cancer Answer each of the following questions from the perspective of the genetic counselor. Question 1: My doctor told me that I have breast cancer, but no one in my family has ever had breast cancer? Why did I get it? I thought that breast cancer was inherited. She told me that it is due to changes in my genes, but I don’t understand how changes in my genes can cause cancer. Question 2: My doctor told me that my tumor makes a high level of Her-2 proteins and that she is going to treat me with a drug called Herceptin®. How does this drug work? My friend had her cancer treated with tamoxifen, and it seems to be working for her. Why can’t I be treated with the same drug?