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Assignment 4 DNA -> Protein ( Translation ) There are 64 codons which code for 20 different amino acids

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Assignment 4

DNA -> Protein ( Translation ) There are 64 codons which code for 20 different amino

acids. Amino Acids join to form a protein sequence. There are 6 reading frames ( 3

forward and 3 reverse) in DNA. All these codes for different protein sequences.

Reference1: Color usage syntax in python

Optional

— OM oF

Separators Sto

_fseseol_| sme |

ays

Can be any of the Can be any of the Foreground (Text) Colors Background Colors

Following ESCAPE CODES: following TEXT STYLES: — Ranges from 30 to 3t Ranges from 40 to 47

Hexadecimal: \x‘bl Normal: 0 Black: 30 Black: 40

Unicode: \u001b/ Bold: 4 Red: 34 Red: U4

Octal: \033l Light: 2 Green: 32 Green: 42

Italicized: 3 Yellow: 33 Yellow. 43

Underlined: 4 Blue: 34 Blue: 44

Blink: 5 Purple: 35 Purple: 45

Cyan: 36 Cyan: 46

White: 3 White: ut

Or you can use colorama python lib.

>>> print(‘\033[1;30;43mA\033[0;0m\033[1;30;42mT\033[0;0m')

AT

a en ee nn cae

A

Reference 2:Codon Table (genscript.com)

Write a PYTHON program to generate 192 bases(characters) using random of “ATGC”

1. Read the DNA sequence in all the 6 reading frames and print their corresponding

protein sequence.

Forward frame 1

Exon sequence: ATGGAG GAGCCG CAG TCAGAT CCI AGC GIC GAG CCC CCT CIG AGT CAG GAAACA TTT TCAGACCTAI

AAsequence MQ P Ms MPs MDP oP Os Gs

Forward frame 2

Exon sequence: GAG GAG CCG CAG TCAGAT CCT AGC GTC GAG CCC CCT CTG AGT CAG GAA ACA TTT TCAGACCTATGGA

AA sequence: [I s ER s ES <s Es ee I

2 } Print the number of start (ATG) and stop codons (TAA, TAG, TGA) and their

positions in all the six reading frames

3. Translate the DNA sequence from the start codon

Note:

64 Codons = 64 x 3 = 192 Amino acids

ATGCAATTGGCC............ GIGATT (192 bases)

Forward 1...192

Reverse 192..1

Alanine (A)

Arginine (R)

Asparagine (N)

Aspartate (D)

Cysteine (C)

CTU Tear hay

Glutamine (Q)

ey lefl alma

Isoleucine (I), Leucine (L

Tal)

Proline (P)

Expected output

Forward:

Dna seq:

ACC AAG GTA ACA TGT CTC TAT CTC CCG TIT GCT TAT TTA TCC CTC CCA ATT GGA GGC TAC TAC AG GCC TGG TCA CTG

T AAA GG ACA GCA TGT GGT TCT CTA GAA GCA AGT TTC CTG CAA TAG CAA TGT GGC CTC GC TTA TAG GCG ATC ACT CE

AGC TAC CCT AAA AGT CCC GGT

protein:

he

| omen T- T H R S ae |

Dna seq:

AAG GTA ACA TGT CTC TAT CTC CCG TTT GT TAT TTA TCC CTC CCA ATT GGA GCC TAC TAC AGC GC TGG TCA CTG CCT

A GCG ACA GCA TGT GGT TCT CTA GAA GCA AGT TTC CTG CAA TAG CAA TGT GCC CTC GCC TTA TAG GCG ATC ACT CAC AG

TAC CCT AAA AGT CCC GGT

protein: — a os a

is TI¢ 3 SMe. s Lee 6d URS 6 OO:

S Qo o/c T H R S PK s Bp

Reverse:

[na seq:

TGG CCC TGA AAA TCC CAT CGA CAT GGA CAC TCA CTA GCG GAT ATT CCG CTC CG TGT AAC GAT AAC GTC CTT TGA ACG

T TGG TGT ACG ACA GCG AAA TCC GTC TCC GTC ACT GGT CCG CGA CAT CAT CGG AGG TTA ACC CTC CCT ATT TAT TCG T

TAT CTC TGT ACA ATG GAA CCA

protein:

mole nue H Ss D iy R GN DN T «K sw C|T T |

P/R H H RR vy 3 S c|T E|®

Dna seq:

TGG CCC TGA AAA TCC CAT CGA CAT GGA CAC TCA CTA GCG GAT ATT CCG CTC CGG TGT AAC GAT AAC GTC CTT TGA ACG

T TGG TGT ACG ACA GCG AAA TCC GTC TCC GTC ACT GGT CCG CGA CAT CAT CGG AGG TTA ACC CTC CCT ATT TAT TCG T

TAT CTC TGT ACA ATG GAA