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Suppose you generated Hfr E

Biology Apr 09, 2022

Suppose you generated Hfr E.coli cells to determine the bacterial gene order, Next, assume that you perform several parallel conjugation experiment, which are dispute using a conjugation inhibitor. after cells allowed to recover, you analyze the genetic makeup of the recipient cells. Here's the data you collect: 1) recipient bacteria that conjugated for 5 minutes became Tra+; 2) After 25 minute recipients became Cys+, Tra+ , and Suc+; 3) after 15 minutes, recipient cells became Tra+ and Cys+; and4) after 30 minutes, recipient cells became Suc+, Cys+, and Tra+ and His +

a. what's the F facter status of the cells that you used in your experiment?

b. Propose a simple plausible model for the conjugation inhibitor,in other words, what must it accomplish

c. based on the given information, derive the gene order for the E. coli genome.

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