1. Which of the following statements is FALSE?

In fetal hemoglobin the central cavity is lined with more histidines than in adult hemoglobin, which results in preferential stabilization of the R-state.

2. Which of the following statements is FALSE with respect to hemoglobin's transition from the T state to the R state?

Helix F changes its secondary structure in response to oxygen binding.

3. Which of the following statements about 2,3 bisphosphoglycerate (BPG) is TRUE?

The affinity of BPG binding to Hb would be reduced if the N-terminal groups of the four subunits were modified to make them uncharged

4. During vigorous exercise, the pH of blood passing through skeletal muscle decreases. How does this decrease affect the behaviour of hemoglobin?

It decreases O2 binding to hemoglobin, because it increases the binding of BPG

5. The enzyme 2,3-BPG mutase produces 1,3-BPG in red blood cells. Which of the following amino acids would you predict to find in the active site of 2,3-BPG mutase?

Arg

6. Which of the following is not a ligand to the porphyrin ring Fe(II) ion in oxymyoglobin?

His E7

7. Which gas does not bind to the porphyrin ring Fe(II) ion in myoglobin?

CO2

8. Which of the following statements does not apply to the K value in the equation for the oxygen binding curve of myoglobin?

If Y > K, then myoglobin is less than 50% saturated with oxygen.

9. Myoglobin's secondary structure is primarily composed of ______________.

alpha helices

10. Myoglobin's primary physiological role is to facilitate oxygen ________.

diffusion

11. If the gene for myoglobin is "knocked out" in mice, the mice:

appear normal, with lighter colored muscle tissue

12. Carbon monoxide binds to heme:

with a higher affinity than oxygen.