You perform this PCR reaction using genomic DNA from tumors isolated from 4 different patients and your results are shown below

You perform this PCR reaction using genomic DNA from tumors isolated from 4 different patients and your results are shown below. Provide a likely explanation for the results shown for each patient. 1— N (‘0 tr Patient1: 5 if, E 5 ‘5 “c6 ‘5 E M n. o. o. a. Patient 2: 1000 Patient 3: 500 100 Patient4: 10 4d. If you designed your primers to immediately ?ank the transposon, what problem would you encounter? 5. Explain why RNA primers are not used in PCR.

6. If the below DNA undergoes a double stranded break as shown, how will this be repaired if a homologous template is not available? What will the sequence of the repaired DNA be? 5 ' -AATGATCCGACC-3' 3 ' -TTACTAGGCTGG-5' 5 ' -AATGAT CCGACC-3' 3' -TTA TGG-5' 7. List all of the possible DNA repair mechanisms (base excision, mismatch, nucleotide excision, end-joining, homologous recombination) described by the following: a. Uses DNA Pol Beta b. Involves the removal of multiple nucleotides c. Ligase is needed d. Requires a template