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I. Answer the following questions based on the plot below.

1. What kind of plot is depicted (ie Lineweaver-Burk, etc)?
2. What kind of inhibitor is present? How do you know (include Km and Vmax within your discussion)?

2. IfVmax = 100gmol/mL sec and Km = 2mM, what is the velocity of the reaction when [S] -20 mM. Watch units.
3. Triose phosphate isomerase catalyzes the conversion of glyceraldehydes-3-phosphate to dihydroxyacetone phosphate.

Glyceraldehydes-3*P        dihydroxyacetone-P

The Km of this enzyme for its substrate glyceraldehyde*phosphate is 1.8 x 10" M. When [glyceraldehydes*phosphate] = 301.1M, the rate of the reaction, v, was 82.5 umol/mL sec.

a. What is Vmax for this enzyme?

b. Assuming 3 nanomoles per mL of enzyme was used in this experiment ([EtotaI]

nanomol/mL), what is kcat for this enzyme?

What is the catalytic efficiency (kcat/Km) for triose phosphate isomerase?

4. A student conducted 3 kinetic experiments using enzyme "B". Based on the data below, please answer the following questions.
5. Is AMP an allosteric activator or inhibitor? Explain. Include in your answer information pertaining to Km (does it change? Why? If so, what does this change indicate?),
6. b. Is ATP an allosteric activator or inhibitor? Explain. Include in your answer information pertaining to Km (does it change? Why? If so, what does this change indicate?).