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Florida International University PCB 3063 15

Biology May 04, 2021

Florida International University

PCB 3063

15.1Eukaryotic Transcription

1)What is the most important difference between prokaryotes and eukaryotes in the process of transcription?

  1. How does this impact the eukaryotic cell?
  2. What is the difference in the eukaryotic polymerases?
  3. What does the term monogenic mean?
  4. What are transcription factors?
  5. Where is RNA polymerase located?
  6. Why are the rRNA molecules considered structural RNAs?
  7. The rRNAs are components of what?
  8. Which polymerase synthesizes all of the rRNAs except for the 5S rRNA molecule?
  9. What is the location and function of the RNA polymerase II?
  10. What has to happen to eukaryotic pre-mRNAs before translation?
  11. RNA polymerase II is responsible for what?
  12. What is the location and function of RNA polymerase III?
  13. What is the role of tRNAs in translation?
  14. Describe the TATA box for the mouse thymidine kinase gene.
  15. What happens after a generalized promoter of a gene transcribed by RNA polymerase II is present?

 

  1. What happens to the introns contained in eukaryotic mRNA and what has to be added to the mRNA afterwards?
  2. What are pseudogenes?
  3. What components help regulate the frequency with which pre-mRNA is synthesized from a gene?
  4. What is the role of enhancers and silencers?
  5. What is the role of basal transcription factors?

Eukaryotic Elongation and Termination

  1. Following the formation of the preinitiation complex, what happens?
  2. When eukaryotic cells are not dividing, there genes exist as what?
  3. What are nucleosomes?
  4. For polynucleotide synthesis to occur, the transcription machinery needs to move what?
  5. What structure accomplishes this?
  6. In eukaryotes, elongation by RNA polymerase II takes place where?

 

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