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Answer the following questions in complete sentences

Biology

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Answer the following questions in complete sentences. Write your answers in black text and leave the questions in blue. All of my constructive critiques will be in red and returned for you so you can study this for the final. Include all relevant data and use 12 fonts single spaced. Include all sequence data regardless of how many pages it turns out to be. Use supplemental texts Alpha1, Beta1, Gamma1, Delta1, Delta2, and Epsilon1.    

 

1. Describe the lifecycle of Ribeiroia ondatrae. Postulate how such a lifecycle evolved on a molecular level. Also include pictures which you have drawn illustrating the lifecycle. Be as descriptive as possible. (10Pts)

 

2. Describe the single celled Eukaryote with GenBank assembly accession number GCA_000499105. Where is it found? What pathologies does it cause? What is its nickname? Is it dangerous? Why? Explain on a molecular level how or why these bacteria are dangerous. How does it damage your physiology? (10Pts)

 

3. Refer to supplementary file Alapha1. Identify the genome and then write a few paragraphs summarizing the strain to which the sequence belongs. Include symptoms, treatments, and discuss any related strains you may find. (10Pts)

 

4. Refer to the supplementary file Beta1. First, identify each of the four sequences. Next run a phylogenetic analysis and make a phylogenetic tree showing how related each sequence is to each other. Discuss evolutionary relationships between the sequences. Be sure to include the alignments, which show point mutations or gaps between the sequences. (10Pts)

 

5. Refer to the supplementary file Gamma1. Identify the sequence and then highlight the motifs, which are present. (10Pts)

 

6. Refer to supplementary file Epsilon1. Identify the sequence and then identify any motifs if any found on it. Include the analysis in your report that highlights the motifs.  (10Pts)

 

7. Refer to supplementary file Phi1, identify the sequence, and then view the multi-sequence alignment viewer (MSA) and show all of the discrepancies between various strains which have been sequenced. (10Pts)

 

8. Refer to sequence Sigma1 and identify the sequence and identify how many proteins are found within it. (10Pts)

 

9. Explain in your own words how PCR works and describe the entire protocol and how the data would be interpreted. (10Pts)

 

10. Explain the process of genetic sequencing using modern sequencers (Ion torrent, or SMRT sequencer). (10Pts)