Place the events that take place during translation and protein synthesis in the correct order

The events that take place during translation are as under:

1) The smaller subunit of the ribosome attaches to the mRNA strand and moves along with it: On completing a cycle of polypeptide synthesis the 30S and 50S subunits remain associated as an inactive 70 S ribosome. Initiation factor 3 binds to the 30S subunit of the comples so as to promote dissociation. Subsequently, IF2 GTP fmet tRNA f met and IF3/MRNA 30S complexes combine with each other to form 30S initiation complex. It moves along the mRNA until it finds a start codon.

2) The anticodon of a tRNA molecule carrying an amino acid matches with the complementary codon on the mRNA strand: This stage is known as decoding in which a ribosome selects and binds an amino acyl tRNA, whose anticodon is complementary to the mRNA codon in the A site.

3) The ribosome adds a new amino acid to a growing polypeptide: The amino acid carried by the tRNA in the P site is transferred to the amino acids in the A site . The polypeptide increases in length as the ribosome adds a new amino acid.

4) The tRNA molecules that have contributed amino acids to the protein chain leave the ribosome: The tRNA in the P site is moved to the E site and then is released.This is known as translocation.