What is the purpose of Coomassie Blue Dye and the acetic acid solution used in lab 7 for protein SDS-PAGE?  Group of answer choices None of those   all of those   •Stain the gel in Coomassie Blue and Destain it in acetic acid solution (destain solution)   •destain the gel in Coomassie Blue after staining it in acetic acid solution (destain solution)     Flag question: Question 8Question 81 pts   You do not need to use a  new pipette tip on the micropipette every time you get a new sample

What is the purpose of Coomassie Blue Dye and the acetic acid solution used in lab 7 for protein SDS-PAGE? 

Group of answer choices

None of those

 

all of those

 

•Stain the gel in Coomassie Blue and Destain it in acetic acid solution (destain solution)

 

•destain the gel in Coomassie Blue after staining it in acetic acid solution (destain solution)

 

 

Flag question: Question 8Question 81 pts

 

You do not need to use a  new pipette tip on the micropipette every time you get a new sample.

Group of answer choices

False

 

True

 

 

Flag question: Question 9Question 91 pts

 

We always use a new pipette tip on the micropipette every time we get a new sample to avoid contaminating the other samples.

Group of answer choices

False

 

True

 

 

Flag question: Question 10Question 101 pts

 

DNA fingerprinting works because:

Group of answer choices

None of those

 

• All organisms contain RNA

 

• The most important genes are different among most people

 

• Most genes are dominant

 

• No two people, except identical twins, have exactly the same DNA

 

 

Flag question: Question 11Question 111 pts

 

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

Group of answer choices

None of those

 

• the agarose gel is positively charged

 

• DNA is positively charged to attracted to the negative end of the unit

 

• the agarose gel in negatively charged

 

• DNA is negatively charged so attracted to the positive end of the unit

 

 

Flag question: Question 12Question 121 pts

 

The principle of agarose electrophoresis is that charged particles will move toward electrodes that have the same charges under the influence of an electric field

Group of answer choices

False

 

True

 

 

Flag question: Question 13Question 131 pts

 

Tallness (T) is dominant to shortness (t) in pea plants. Which of the following represents a genotype of a pea plant that is heterozygous for tallness?

Group of answer choices

tt

 

TT

 

Tt

 

None of those

 

 

Flag question: Question 14Question 141 pts

 

Tallness (T) is dominant to shortness (t) in pea plants. Hemoglobin A (Hb A)  is dominant to Hemoglobin S (Hb S) in sickle cell disease genotyping. Select all Recessive traits that apply below

Group of answer choices

TT

 

Hb A/Hb S

 

Hb S/Hb S

 

Tt

 

Hb A/Hb A

 

tt

 

 

Flag question: Question 15Question 151 pts

 

Tallness (T) is dominant to shortness (t) in pea plants. Hemoglobin A (Hb A)  is dominant to Hemoglobin S (Hb S) in sickle cell disease genotyping. Select all dominant traits that apply below

 

Group of answer choices

TT

 

Hb A/Hb A

 

Tt

 

Hb A/Hb S

 

Hb S/Hb S

 

None of those

 

tt

 

 

Flag question: Question 16Question 161 pts

 

If the Black phenotype is dominant then its genotype will be

Group of answer choices

BB

 

bb

 

Bb

 

None of those

 

b

 

D

 

 

Flag question: Question 17Question 171 pts

 

Variant forms of a given gene are known as ________________

Group of answer choices

allele

 

trait

 

None of those

 

phenotype

 

gene

 

genotype

 

 

Flag question: Question 18Question 181 pts

 

On what basis are hemoglobin A and S separated in lab 9? 

Group of answer choices

None of those

 

b. Mass

 

a. Charge

 

c. charge and Mass

 

 

Flag question: Question 19Question 191 pts

 

What is the condition known for a person with HbA/HbS genotypes?

Group of answer choices

sickle cell trait

 

None of those

 

sickle cell

 

normal

 

 

Flag question: Question 20Question 201 pts

 

What amount of agar do you need to weigh if you want to make 50mL of a 2% (w/v) agarose gel?

Group of answer choices

2g

 

0.2g

 

50mg

 

None of those

 

5mg

 

 

Flag question: Question 21Question 211 pts

 

What's the purpose of DNA extraction?

Group of answer choices

c- Lyse the cells in a way that the DNA get released

 

a- Force the cell to make more DNA

 

d- Make the cells release DNA in vesicles

 

None of those

 

b- Kill the cells.

 

 

Flag question: Question 22Question 221 pts

 

What is the purpose of the ladder when loading a PCR gels?

Group of answer choices

d) Identify how pure the DNA is.

 

c) Just for fun

 

b) Being able to identify the weight of the bands

 

a) Positive control for running the gel

 

all of those

 

None of those

 

 

Flag question: Question 23Question 231 pts

 

Select all of the ingredients that are required for PCR experiments.

Group of answer choices

e) dNTP

 

a) Taq polymerase

 

b) SYBR green master mixte

 

f) Ethidium bromide

 

d) primers

 

i) None of those

 

g) Agarose

 

h) Coomassie blue

 

c) SDS

 

 

Flag question: Question 24Question 241 pts

 

In lab 10, What is the role of Ca2+ ions (calcium) in the production of α-Amylase

Group of answer choices

Ca2+ ions activate Gibberellin (GA) activity

 

Ca2+ ions inhibit Gibberellin (GA) activity

 

Ca2+ ions are Co-factor of amylase.

 

None of those

 

Ca2+ ions inhibit amylase activity.

 

 

Flag question: Question 25Question 251 pts

 

Abscisic Acid (ABA) inhibits the GA-dependent synthesis of hydrolytic enzymes

Group of answer choices

False

 

True

 

 

Flag question: Question 26Question 261 pts

 

Gibberellin (GA) Activates the Production of α-Amylase

Group of answer choices

True

 

False

 

 

Flag question: Question 27Question 271 pts

 

Gibberellin (GA) inhibites the Production of α-Amylase

Group of answer choices

True

 

False

 

 

Flag question: Question 28Question 281 pts

 

What would you do if you have to take 1,030 µl? Which pipettor(s) should you use 

Group of answer choices

None of those

 

P1000

 

P200

 

P1000 and P20

 

P200 and P20

 

P1000 and P200

 

 

Flag question: Question 29Question 291 pts

 

What weight of agarose in g would you use to make a 2.75% agarose gel in 20ml total volume? 

Group of answer choices

0.55g

 

0.55mg

 

0.75mg

 

0.5g

 

0.75g

 

0.25g

 

 

Flag question: Question 30Question 301 pts

 

What is the function of Ethidium Bromide?

 

Group of answer choices

Ethidium Bromide binds to  DNA so that the DNA banding pattern can be visualized under direct visible light source.

 

Ethidium Bromide binds to  DNA and RNA so that they can be visualized under a UV light source.

 

None of those

 

Ethidium Bromide binds to  DNA so that the DNA banding pattern can be visualized without any light source.

 

Ethidium Bromide binds to  DNA so that the DNA banding pattern can be visualized under a UV light source.