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The goal is to write a report on an unknown project did, we got a list of a bunch of bacterias and did a bunch of tests to try to figure out what the bacteria is

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The goal is to write a report on an unknown project did, we got a list of a bunch of bacterias and did a bunch of tests to try to figure out what the bacteria is. My unknown bacteria was Enterbacter aerogenes I found this out by first finding out the gram stain of my bacteria my unknown bacteria was a negative gram stain so I crossed out the ones that were positive. I then went over the shape of the bacterias. My unknown was rod shaped so I crossed out the ones that weren't. I then did the sheep blood agar experiment , MaConkey experiment , and the TSI experiment . I then found out my bacteria name.Bacterial Unknowns Project Introduction For this project, each student will be given one numbered test tube containing a culture of either a Gram positive organism or a Gram negative organism. The cultures the students will consist of any of the following organisms. Gram Positives: Bacillus cereus Bacillus subtilis Corynebacterium xerosis Lactococcus lactis Staphylococcus epidermidis Streptococcus pyogenes Staphylococcus aureus** Gram Negatives: Alicaligenes faecalis Citrobacter freundii Enterobacter aerogenes Escherichia coli Neisseria sicca Providencia stuartii Pseudomonas aeruginosa** Students will demonstrate the correct execution of fundamental microbiology laboratory technique and use a multitude of differential tests to determine and staining techniques to identify of their unknown organisms. Materials & Resources On the first day of the investigation, each student will be given two nutrient agar plates. Since each student will be designing their own project, not everyone in the class will be completing the same tests at the same time. Given limited materials, students (in general) should provide the instructor with a list of materials at least 48 hours before attempting to conduct a desired test. All students will be allowed to use any of the media located in the refrigerator of the classroom. Students should also refer to Bergey’s Manual as a resource for determining the standards for differential tests of the potential organisms and the student’s individually constructed diagnostic standard matrix. Laboratory Attendance Students will have several class lab periods to work on their investigation. It is the responsibility of each student to come to lab with a clear investigation plan and a list of tests and research methods the student will be working on that day. Students will be required to sign in to lab during each of these lab days. Instructor Role During these investigations, the instructor will serve as an observer providing the student with guidance throughout the investigation as requested. The instructor is able to assist students in the proper execution of lab technique, waste disposal instructions, suggestions for trouble shooting during the project, and in the acquisition of differential test resources. The instructor is not to be used as a database for confirmation of diagnostic standards for specific organisms or for the unknown organisms. Students may ask the instructor only once for confirmation of the Gram negative organism and the Gram positive organism. Grading This project is worth 10 % of the student’s overall course grade and is comprised of the following components. Unknown’s Project Grade: In-class Investigation: 50% During the Unknown Project, students will be assessed on their performance in the laboratory. Students will be graded on the following: a) Experimental Design and Project planning b) Adherence to BSL-2 Laboratory Safety regulations (includes wearing of PPE). c) Execution of proper laboratory techniques such as aseptic transfer, streak plate, staining techniques, use of microscopes etc. d) Communication with the laboratory instructor. e) Proper disposal of hazardous material. Microbiology Formal Lab Report: 50% Microbiology Formal Lab Report Criteria After the completion of identifying the unknown specimens, students will be required to construct a formal lab report based on the following criteria. Students are to work independently of one another as this is not a group assignment. General Format (4 points) The report must consist of the following components: Cover Page, Title, Introduction, Methods, Results, Discussion, Conclusion, References, and Flow Chart. Each section should be clearly labeled with its appropriate heading outlined in bold. For example the Introduction Section must be labeled “Introduction”. Each section must be presented in paragraph form. The beginning of each new paragraph should start with a line that is indented (see the first line of this paragraph). A paragraph is defined as a text of information involving 5 or more sentences. Reports must be typed using Times New Roman Font and size 12 text. Page margins must be 1 inch by 1 inch. A header including the student’s last name followed by his/her first initial and the page number of the report must be placed in the upper right hand corner of each page (Ex. James A. pg 5). Also required is a cover page containing the following information presented in the following order: Title, Student Name, Instructor Name, Course Name, Section, Date.. *Any portion of the paper reflecting plagiarized material will result in a grade of zero for the overall report. General Writing Style (4 points) Clarity Each sentence presented within the lab report should be clearly stated. Vague descriptive terms such as “good” or “kind of” should be avoided. All conclusions present within the report should be based solely on scientific data, facts, and observations. The paper should be written in a scientific manner. Spelling of Organisms All 10 plausible organisms should be completely written out when first addressed in the Introduction. The use of the abbreviated names is applicable throughout the remainder of the paper (example: P. aeroginosa). All organism names completely written out (ex. Micrococcus roseus) and abbreviated (example: P. vulgaris) need to be outlined in italics with the first letter of the genus name capitalized and the first letter of the scientific epithet lower cased. Title (2 pt) The title must be clearly conveyed on the cover page of the report and must describe the scope and purpose of the report. Example Title: The Identification of Two Unknown Species of Bacteria in tubes #99 Introduction (10 pts) In the introduction the student must provide some background information on why it is important to be able to determine unknown microbial organisms and what information can be taken from performing such an investigation. The student must also address and answer the following questions within the introduction of the paper: What problem is being investigated? In general, how will this investigation be performed, and why is it important for such an investigation to be performed? What types of tests will be utilized in the experiment? Why are biochemical tests being used? What are the possible organisms the investigator may have within his/her unknown sample? What information does the investigator know for certain before the execution of the investigation? Methods (10 points) In this section, it is the student’s duty to clearly explain what tests and protocols are being performed throughout the investigations. Considering the numerous number of tests being employed, students should label each test/procedure performed and clearly outline the protocols for each test. Students must cite the lab manual as a reference for the all tests performed following the protocols as specified in the lab manual. See reference section for examples on how to cite references within the paper. Results (20 points) The results section should be presented using charts, drawings, diagrams, pictures, and tables. Each figure included in this section must contain a title, a caption, and a figure number. Tables should be referred to as “Tables” and must also be assigned a number. Results of all tests performed such as gram staining, colony morphology data collection, biochemical tests, etc. must all be presented clearly in this section. See examples below. Example of caption for a picture. Figure 1. The above figured depicts the results from the Litmus Test after a 2 week incubation period. Discussion (25 points) This section is the most crucial portion of the lab report. In this section students will need to walk through the process of how each of the 10 plausible organisms were eliminated using gram staining techniques, biochemical tests, colony morphology comparisons, etc. Students should evaluate the data presented in the results section of the paper and explain the data’s significance in determining the identification of the two unknowns present in the sample. In this section the student needs to prove how the results of each test performed support the identification of each organism. Students to perform outside research and acquire information regarding the pathogenic nature of each organism identified as well as explain specific characteristics of each organism such as the optimal environmental growth conditions required for each. Students should clearly refer to their 2 organisms as “Unknown A” and “Unknown B”. Students must also clearly explain which unknown is gram positive and which the gram negative organism is. Conclusion (10 points) In this section, students are to clearly state the identity of organisms, Unknown A” and “Unknown B”. The names of each organism must be written in italics with the genus name capitalized and the scientific name in all lower case letters (e.g. Staphylococcus epidermidis). *Points will be deducted for improper writing format of organism name. References & In -text Citations (5 points) A minimum of 3 references should be cited in this section. Bergey’s Manual of Definitive Microbiology, the course’s Microbiology Lab Manual and an additional source should be cited in this section. Other sources that are used for reference can include the textbook, websites, and other sources. References must be listed in numerical order as they appear in the report. Use APA citation formation using superscript numbers at the end of sentences citing references. Example of citing a book source within the methods section of the lab report: After obtaining a starch agar plate and inoculating the plate with B. subtilis and E. coli, the plates were left to incubate for at least 48 hours at 37 degrees Celsius1. Example of listing a book reference in the reference section of the paper: [1] Author’s Last name, First Initial (highlight in bold). Title of book in italics. Name of Publisher Company. Year of publication. Pages numbers. Example: [1] Sigmon, J. Bio 260: Microbiology Lab Manual. Marymount University. 2004. pp.1-61. Flow Chart (10 points) A flow chart outlining the process of elimination of each individual organism must be provided clearly detailing the investigation. This is added in the lab report as Appendix A. See Example on page. Flow Chart Example UI WIIS Troject Introduction For this project, each student will be given one numbered test tube containing a cu either a Gram positive organism or a Gram negative organism. The cultures the students consist of any of the following organisms. Gram Positives: Bacillus cereus Bacillus subtilis Corynebacterium xerosis Lactococcus lactis Staphylococcus epidermidis Streptococcus pyogenes Staphylococcus aureus** Gram Negatives: Alicaligenes faecalis Citrobacter freundii Enterobacter aerogenes Escherichia coli Neisseria sicca Providencia stuartii Pseudomonas aeruginosa** Students will demonstrate the correct execution of fundamental microbiology laboratory technique and use a multitude of differential tests to determine and staining techniques to identify of their unknown organisms. 1 A P SAN MacBook Pro PYREX HUU . #10 Ashebin Naomi (???