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PROTOCOL FOR REVERSED-PHASE SEPARATION USING CYCLOBOND SN/ RN Weak acid or base Optimize selectivity Strong interaction with stationary phase Strong interaction with stationary phase Optimize selectivity If separation is not achieved under reversedphase mode, switch to normal phase mode Change column type PROTOCOL FOR NORMAL-PHASE SEPARATION USING CYCLOBOND SN/ RN Solvent strength is OK but needs improvement in selectivity (test different selectivity parameters) Increase solvent strength i
PROTOCOL FOR REVERSED-PHASE SEPARATION USING CYCLOBOND SN/ RN Weak acid or base Optimize selectivity Strong interaction with stationary phase Strong interaction with stationary phase Optimize selectivity If separation is not achieved under reversedphase mode, switch to normal phase mode Change column type PROTOCOL FOR NORMAL-PHASE SEPARATION USING CYCLOBOND SN/ RN Solvent strength is OK but needs improvement in selectivity (test different selectivity parameters) Increase solvent strength i.e., 100% ACN (polarorganic mode) i.e., solvent type For acid For base Even in NP mode, optimize resolution (1
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