Homework answers / question archive / 1_)What is the buffer condition required for polypeptide N-terminal sequencing using Sanger's reagent (Dansyl chloride) or Edman's reagent (phenyl isocyanate, PITC) to label the first amino acid residue of a polypeptide to be sequenced? 2)A

1_)What is the buffer condition required for polypeptide N-terminal sequencing using Sanger's reagent (Dansyl chloride) or Edman's reagent (phenyl isocyanate, PITC) to label the first amino acid residue of a polypeptide to be sequenced? 2)A

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1_)What is the buffer condition required for polypeptide N-terminal sequencing using Sanger's reagent (Dansyl chloride) or Edman's reagent (phenyl isocyanate, PITC) to label the first amino acid residue of a polypeptide to be sequenced?

2)A.Dansyl chloride requires mild acidic condition and PITC mild alkaline condition

1. B.Both require mild alkaline conditions so that the primary amine is deprotonated.
2. C.PITC requires acidic condition and Dansyl chloride alkaline condition
3. D.Both require the existence of a reducing agent to break down disulphide bonds
4. E.Both require mild acidic conditions so that the primary amine is protonated but does not hydrolyze the peptide bond.
5. F.Both require a chaotropic reagent to unfold the polypeptide