Homework answers / question archive / Which reagent reduces disulfide bonds to sulfhydryl groups? Which chemical reagent promotes peptide bond cleavage on the C-side (carboxyl side) of Met residues? Identify the least conservative amino acid substitution, assuming that these two residues occur at the same position in two homologous proteins

Which reagent reduces disulfide bonds to sulfhydryl groups? Which chemical reagent promotes peptide bond cleavage on the C-side (carboxyl side) of Met residues? Identify the least conservative amino acid substitution, assuming that these two residues occur at the same position in two homologous proteins

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1. Which reagent reduces disulfide bonds to sulfhydryl groups?
2. Which chemical reagent promotes peptide bond cleavage on the C-side (carboxyl side) of Met residues?
3. Identify the least conservative amino acid substitution, assuming that these two residues occur at the same position in two homologous proteins.
4. The amino acid sequence of which type of protein is likely to have changed the least during evolution?
5. Which of the following statements is not true about the Bradford assay?
6. Which of the following statements is not true about SDS-PAGE:
7. Which of the following statements about affinity chromatography is not true:
8. Which chemical reagent promotes peptide bond cleavage on the C-side (carboxyl side) of lysine and arginine residues if the next residue is not proline?
9. What would be the order of elution (from first --> last) of the proteins carbonic anhydrase (pI = 7.0), carboxypeptidase B (pI = 6.2) and chymotrypsin (pI = 8.0) run on a CM (carboxymethyl) ion exchange column at pH 6.6 eluted with an increasing NaCl gradient:
10. Ion-exchange chromatography