which of these techniques is used to separate proteins mainly based on mass Disfulfide bonds can be cleaved using which of the following reagents is commonly used to determine the number of polypeptides in a protein enzymes that hydrolyze the internal peptide bonds of a protein are classified as which of the following substances cannot be used to cleave peptide bonds in polypeptides Which of these are commonly used to cleave peptide bonds in polypeptides? The peptide Leu?Cys?Arg?Ser?Gln?Met is subjected to Edman degradation

1. which of these techniques is used to separate proteins mainly based on mass

SDS-PAGE

2. Disfulfide bonds can be cleaved using

2-mercaptoethanol

3. which of the following reagents is commonly used to determine the number of polypeptides in a protein

dansyl chloride

4. enzymes that hydrolyze the internal peptide bonds of a protein are classified as

endopeptidases

5. which of the following substances cannot be used to cleave peptide bonds in polypeptides

2-mercaptoethanol

6. Which of these are commonly used to cleave peptide bonds in polypeptides?

trypsin

7. The peptide Leu?Cys?Arg?Ser?Gln?Met is subjected to Edman degradation. In the first cycle the peptide first reacts with phenylisothiocyanate under basic conditions. The product of this reaction is incubated with anhydrous trifluoroacetic acid and subsequently with an aqueous acid. What are the products generated in the first cycle.

PTH?Leu and Cys?Arg?Ser?Gln?Met

8. Edman degradation can be used to

identify the n-terminal amino acid of a polypeptide

9. The positive charge on proteins in electrospray ionization mass spectrometry is the result of

protonated side chains of Arg and Lys residues

10. ___ has emerged as a technique for protein sequencing

Mass spectrometry