HPLC stands for a technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called a technique that can be used to separate proteins based on their pI is called which of the following amino acids would be last to elute at pH 8 from an anion exchange column Which of the following amino acids would be the first to elute at pH 8 from an anion exchange column The pK1, pK2, and pKR of the amino acid lysine are 2

1. HPLC stands for

high performance liquid chromatography

2. a technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called

hydrophobic interaction chromatography

3. a technique that can be used to separate proteins based on their pI is called

isoelectric focusing

4. which of the following amino acids would be last to elute at pH 8 from an anion exchange column

glutamic acid

5. Which of the following amino acids would be the first to elute at pH 8 from an anion exchange column

lysine

6. The pK1, pK2, and pKR of the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The pK1, pK2, and pKR of the amino acid arginine are 1.8, 9.0, and 12.5, respectively. A student at SDSU wants to use ion exchange chromatography to separate lysine from arginine. What pH is likely to work best for this separation?

10.5

7. The pK1, pK2, and pKR of the amino acid histidine are 1.8, 9.3, and 6.0, respectively. The pK1, pK2, and pKR of the amino acid arginine are 1.8, 9.0, and 12.5, respectively. You have a mixture of histidine and arginine, how would you try to separate these two amino acids?

cation exchange chromatography at pH 6

8. What can be done to increase the rate at which a protein of interest moves down an ion-exchange chromatography column?

add a small amount of a non-ionic detergent to the elutant

9. Hydrophobic interaction chromatography can be used to separate proteins based on differences in

polarity

10. You are trying to separate five proteins, which are listed below, by gel filtration chromatography. Which of the proteins will elute first from the column?

glutamine synthetase (621 kDa)