Identify which proteins require pre-treatment with performic acid before amino acid sequencing Identify which proteins would bind to an anion exchange column at pH 7 Identify which proteins would give more than one product after a single cycle of Edman degradation Which of the following is an example of a conservative amino acid substitution in a protein structure Which of the following is not a conservative amino acid substitution in a protein structure Which of the following amino acids is most likely to be a conservative substitution for glycine A fast and common method for determining the protein concentration in column effluent is the quantitation of proteins due to their absorbance at 280 nm is due to the large absorptivity of the _ amino acids 5 Graduate students prepare extracts from 5 different tissues

1. Identify which proteins require pre-treatment with performic acid before amino acid sequencing

3, 4, & 5

2. Identify which proteins would bind to an anion exchange column at pH 7

2 & 4

3. Identify which proteins would give more than one product after a single cycle of Edman degradation

4 & 5

4. Which of the following is an example of a conservative amino acid substitution in a protein structure

Ser to Thr

5. Which of the following is not a conservative amino acid substitution in a protein structure

Arg to Phe

6. Which of the following amino acids is most likely to be a conservative substitution for glycine

Ala

7. A fast and common method for determining the protein concentration in column effluent is

measuring light absorption at 280 nm

8. the quantitation of proteins due to their absorbance at 280 nm is due to the large absorptivity of the _ amino acids

aromatic

9. 5 Graduate students prepare extracts from 5 different tissues. eaxh student measures the total amount of alcohol dehydrogenase and the total amount of protein in his or her extract. Which has the highest specific activity?

B

10. Which physical characteristic is not commonly used in protein separation?

Stereochemistry