Pre-Lab13: Sickle Cell Disease Detection Lab

Question 11 pts

What type of point mutation leads to the development of sickle cell anemia? 

Group of answer choices

silent

conservative missense

nonsense

non-conservative missense

Question 20.5 pts

Given that sickle cell anemia is an autosomal recessive condition, what is the probability that a child born from a woman and man who are both heterozygous for the trait (Ss) will have sickle cell anemia?

Group of answer choices

0%

1/2 or 50%

1/4 or 25%

3/4 or 50%

100%

Question 31 pts

When conducting a restriction digest with the endonuclease Mst II, how many fragments  do you expect to see from a person's PCR product who is homozygous for the normal Hb A gene? 

Group of answer choices

no fragments

2 fragments

3 fragments

1 fragment

Question 41 pts

Since DNA has a strong ________ charge at neutral pH, it will migrate towards the red ________ electrode in the electrophoresis apparatus.

Group of answer choices

negative, negative

negative, positive

positive, negative

positive, positive

Question 50.5 pts

In the gel image below, which DNA fragment is the smallest in size? 

*The electrode orientation is indicated by the red colored "-" and "+" symbols.

Group of answer choices

DNA in Lane B

DNA in Lane C

DNA in Lane A

DNA in Lane D

Question 61 pts

Which of the best describes using a loading dye for gel electrophoresis? 

Chose all that apply. There may be more than one answer.

Group of answer choices

directly binds to DNA

used to visualize the DNA after electrophoresis

does not directly bind to DNA

used to monitor the progress of the DNA during electrophoresis