Why Choose Us?
0% AI Guarantee
Human-written only.
24/7 Support
Anytime, anywhere.
Plagiarism Free
100% Original.
Expert Tutors
Masters & PhDs.
100% Confidential
Your privacy matters.
On-Time Delivery
Never miss a deadline.
University of South Florida, St
University of South Florida, St. Petersburg
PCB 3713L
Exercise 8: Chemical and Physical Processes of Digestion: Activity 3: Assessing Pepsin Digestion of Protein Lab Report
Pre-lab Quiz Results
1)Where in the body does protein digestion begin?
- The substrate for pepsin is
- In this activity the substrate you will be using to detect protein digestion is
- Negative results with the negative controls
- A spectrophometer measures
Experiment Results Predict Question:
Predict Question1: At which pH do you think pepsin will have the highest activity?
Stop & Think Questions:
2. Which of the following tubes are negative control tubes?
3. An optical density greater than zero indicates that
|
Tube No. |
Reagent 1 |
Reagent 2 |
Reagent 3 |
Boiled |
Time |
Temp. |
Optical Density |
|
1 |
Pepsin |
BAPNA |
pH 2.0 |
+ |
60 |
37 |
0.00 |
|
2 |
Pepsin |
BAPNA |
pH 2.0 |
- |
60 |
37 |
0.40 |
|
3 |
Pepsin |
Water |
pH 2.0 |
- |
60 |
37 |
0.00 |
|
4 |
Water |
BAPNA |
pH 2.0 |
- |
60 |
37 |
0.00 |
|
5 |
Pepsin |
BAPNA |
pH 7.0 |
- |
60 |
37 |
0.03 |
|
6 |
Pepsin |
BAPNA |
pH 9.0 |
- |
60 |
37 |
0.00 |
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
- Pepsin would be most active
- Which two tubes validated the results of the experiment?
- With more enzyme activity the optical density
- If pepsin were digesting an actual protein substrate, the product would be
Review Sheet Results
- Describe the effect that boiling had on pepsin and how you could tell that it had that effect.
- Was your prediction correct about the optimal pH for pepsin activity? Discuss the physiological correlation behind your results.
- What do you think would happen if you reduce the incubation time to 30 minutes for tube 5?
Expert Solution
PFA
Archived Solution
You have full access to this solution. To save a copy with all formatting and attachments, use the button below.
For ready-to-submit work, please order a fresh solution below.
