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Texas Tech University ZOOL 2304 Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report Pre-lab Quiz Results 1)Which of the following is/are true of bile?   The substrate used in this simulation is __________   When fatty acids are liberated by lipase, the pH __________   One of the products of the chemical digestion of lipids is ________   Experiment Results Predict Question: Predict Question1: Which tube do you think will have the highest lipase activity?     Stop & Think Questions: 2

Chemistry May 04, 2021

Texas Tech University

ZOOL 2304

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results

1)Which of the following is/are true of bile?

 

  1. The substrate used in this simulation is __________

 

  1. When fatty acids are liberated by lipase, the pH __________

 

  1. One of the products of the chemical digestion of lipids is ________

 

Experiment Results Predict Question:

Predict Question1: Which tube do you think will have the highest lipase activity?  

 

Stop & Think Questions:

2.What does the pH measure?

 

3.Why is it difficult to detect whether lipase is active in tube 5?

 

Tube No.

Reagent 1

Reagent 2

Reagent 3

Reagent 4

Time

Temp.

pH

1

Lipase

Vegetable oil

Bile salts

pH 7.0

60

37

6.21

2

Lipase

Vegetable oil

Water

pH 7.0

60

37

6.72

3

Lipase

Water

Bile salts

pH 9.0

60

37

9.00

4

Water

Vegetable oil

Bile salts

pH 7.0

60

37

7.00

5

Lipase

Vegetable oil

Bile salts

pH 2.0

60

37

2.00

6

Lipase

Vegetable oil

Bile salts

pH 9.0

60

37

8.97

 

Post-lab Quiz Results

 

  1. What is the product of lipase hydrolysis?

 

  1. From your results, which pH is ideal for pancreatic lipase digestion?

 

  1. Which tube confirms that there is no lipase in bile salts or vegetable oil?

 

  1. From your results, where (in theory) would pancreatic lipase be active?

 

 

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