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Texas Tech University ZOOL 2304 Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report Pre-lab Quiz Results 1)Which of the following is/are true of bile? The substrate used in this simulation is __________ When fatty acids are liberated by lipase, the pH __________ One of the products of the chemical digestion of lipids is ________ Experiment Results Predict Question: Predict Question1: Which tube do you think will have the highest lipase activity? Stop & Think Questions: 2
Texas Tech University
ZOOL 2304
Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report
Pre-lab Quiz Results
1)Which of the following is/are true of bile?
- The substrate used in this simulation is __________
- When fatty acids are liberated by lipase, the pH __________
- One of the products of the chemical digestion of lipids is ________
Experiment Results Predict Question:
Predict Question1: Which tube do you think will have the highest lipase activity?
Stop & Think Questions:
2.What does the pH measure?
3.Why is it difficult to detect whether lipase is active in tube 5?
|
Tube No. |
Reagent 1 |
Reagent 2 |
Reagent 3 |
Reagent 4 |
Time |
Temp. |
pH |
|
1 |
Lipase |
Vegetable oil |
Bile salts |
pH 7.0 |
60 |
37 |
6.21 |
|
2 |
Lipase |
Vegetable oil |
Water |
pH 7.0 |
60 |
37 |
6.72 |
|
3 |
Lipase |
Water |
Bile salts |
pH 9.0 |
60 |
37 |
9.00 |
|
4 |
Water |
Vegetable oil |
Bile salts |
pH 7.0 |
60 |
37 |
7.00 |
|
5 |
Lipase |
Vegetable oil |
Bile salts |
pH 2.0 |
60 |
37 |
2.00 |
|
6 |
Lipase |
Vegetable oil |
Bile salts |
pH 9.0 |
60 |
37 |
8.97 |
Post-lab Quiz Results
- What is the product of lipase hydrolysis?
- From your results, which pH is ideal for pancreatic lipase digestion?
- Which tube confirms that there is no lipase in bile salts or vegetable oil?
- From your results, where (in theory) would pancreatic lipase be active?
Expert Solution
PFA
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