Bio Final Exam   What are transgenic animals? What is gene therapy? What are the problems associated with gene therapy? What is DNA profiling? What is DNA profiling used for? What is agarose gel elctrophoresis? What is STR analysis? What is PCR and how can it be used to amplify (make many copies) a DNA molecule? What is taq polymerase and what is special about this enzyme? What is genomics?

1. What are transgenic animals?

Genetically engineered animal or its offspring

2. What is gene therapy?

inserting a missing gene or repairing a defective one in humans by inserting desired gene into host cell

3. What are the problems associated with gene therapy?

short-lived nature of gene therapy, immune response, problems with viral vectors, multi-gene disorders, chance of inducing a tumor

4. What is DNA profiling?

a sample of DNA is run through a laboratory to generate information about the DNA

5. What is DNA profiling used for?

Used for identifying source of sample, or used as base of comparison between two samples

6. What is agarose gel elctrophoresis?

used to separate DNA or RNA molecules by size

7. What is STR analysis?

molecular biology method used to compare specific loci on DNA from two or more samples

8. What is PCR and how can it be used to amplify (make many copies) a DNA
   molecule?

(polymerase chain reaction) - amplifies a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence

9. What is taq polymerase and what is special about this enzyme?

found in bacteria that live in heat conditions. Used to synthesize a new DNA strand from a template. It is very stable at high temperatures

10. What is genomics?

the study of the genomes of organisms