Review Sheet - Lab Practical - Microbiology   Define aseptic technqiues

Review Sheet - Lab Practical - Microbiology

1. Define aseptic technqiues.
2. How does handwashing affect microbial growth?
3. How can you tell the difference between a surface contaminated with bacteria and one that is not?
4. Describe how biohazardous material is discarded.
5. How do you prepare a lab table for microbiological work?
6. How do you sterilize a wire loop for use in the microbiology laboratory?
7. Calculate the % reduction in microbial growth.
8. How do you label a Petri dish?
9. Define colony.
10. Describe the function and location of the ocular, condenser, illuminator, objectives and coarse adjustments.
11. Identify the ocular, condenser, illuminator, objectives, and coarse adjustment in a diagram of the microscope.
12. Compute total magnification.
13. Compute resolving power.
14. Calculate the length of a specimen under various magnifications.
15. What are some differences between the letter e as seen without the microscope and the image when seen with the microscope?
16. How is the size of an image in the microscope influenced by various magnifications?
17. What is the difference between a prokaryotic and a eukaryotic cell?
18. What is the difference between a multicellular and unicellular organism?
19. How is a wire loop sterilized?
20. Describe the shape, size, and color of some bacterial colonies.
21. Classify Rhodospirillum rubrum, Bacillus cereus, Streptococcus salivarius, and Escherichia coli based on their shape and arrangement on a glass slide.
22. What is the purpose of streak plaing?
23. How many red, yellow, white colonies grew in the quadrant that contained the fewest colonies? (PICTURE)
24. How do you prepare a smear?
25. What is the purpose of heat fixation?
26. Tell whether Micrococcus luteus, Bacillus cereus, Escherichia coli are Gram positive or Gram negative. Describe their shape and arrangement.
27. Classify the culture medium as selective, differential or selective/differential based on the type of microbial growth observed on the culture medium.
28. Describe the cultural characteristics of Serratia marcesans and Escherichia coli growing in a deep, broth and slant.
29. Tell whether the following results indicate a positive or negative chemical reaction for the following biochemical tests.
30. Determine whether a certain bacteria is susceptible or resistant to an antibiotic.
31. Measure the diameter of the zone of inhibition to determine the level of susceptibility a particular bacteria has      to an antibiotic.
32. Describe the Kirby Bauer Method used in determining the level of antibiotic sensitivity of selected bacteria.
33. Identify the smallest and largest DNA fragments in an agarose gel matrix.
34. How many restriction sites are there for HinD III and EcoR I in a particular DNA molecule?
35. What is the difference between competent cells that contain pBLU+ and pBLU-?