Homework answers / question archive / A B ? D E F G H Last Name First Name Unknown # Fernandes Foster Gilani Gonzales Hackbarth Hamlin Hartenberger Hastings Hauser Jimenez Khoury Lee Lehman Leodones Macario Manfredi Marcos Kevin Legend Shannon Sophia Jayden Mary Joseph Reina Neil Raul Rachel Sofia Lindsey Karla Denise Ashley Brittany Mallory HPLC-25 HPLC-26 HPLC-27 HPLC-28 HPLC-29 HPLC-30 HPLC-31 HPLC-32 HPLC-33 HPLC-34 HPLC-35 HPLC-36 HPLC-37 HPLC-38 HPLC-39 HPLC-40 HPLC-41 Mobile Phase B Compound 1 Methnaol Impurity-C Methnaol benzonitrile Methnaol propylparaben Methnaol methylbenzoate Methnaol anisole Methnaol benzene Methnaol p-nitrotoluene Methnaol butylparaben Methnaol butyrophenone Methnaol valerophenone Methnaol naphthalene Methnaol p-xylene Methnaol Impurity-F Methnaol hexanophenone Acetonitrile benzylalcohol Acetonitrile ibuprofen Acetonitrile benzonitrile Compound 2 ibuprofen p-chlorophenol methylbenzoate anisole propiophenone p-nitrotoluene p-nitrobenzylchloride butyrophenone toluene naphthalene ethylbenzene Impurity-E Impurity-G propylbenzene acetanilide 3-phenylpropanol p-chlorophenol Compound 3 hexanophenone Impurity-F p-xylene bromobenzene p-chlorophenol p-nitrobenzylchloride diethylformamide n-butylbenzene p-nitrobenzylchloride 3-phenylpropanol butyrophenone heptanophenone naphthalene p-chlorophenol p-xylene propiophenone p-chlorophenol Compound 4 heptanophenone p-chlorophenol 3-phenylpropanol Impurity-G propylbenzene benzene propylbenzene p-nitrobenzylchloride acetanilide Impurity-E valerophenone 3-phenylpropanol p-chlorophenol valerophenone valerophenone Impurity-F ethylparaben As a final virtual lab for the semester I would like each of you to determine the best isocratic separation for a set of four analyte compounds

A B ? D E F G H Last Name First Name Unknown # Fernandes Foster Gilani Gonzales Hackbarth Hamlin Hartenberger Hastings Hauser Jimenez Khoury Lee Lehman Leodones Macario Manfredi Marcos Kevin Legend Shannon Sophia Jayden Mary Joseph Reina Neil Raul Rachel Sofia Lindsey Karla Denise Ashley Brittany Mallory HPLC-25 HPLC-26 HPLC-27 HPLC-28 HPLC-29 HPLC-30 HPLC-31 HPLC-32 HPLC-33 HPLC-34 HPLC-35 HPLC-36 HPLC-37 HPLC-38 HPLC-39 HPLC-40 HPLC-41 Mobile Phase B Compound 1 Methnaol Impurity-C Methnaol benzonitrile Methnaol propylparaben Methnaol methylbenzoate Methnaol anisole Methnaol benzene Methnaol p-nitrotoluene Methnaol butylparaben Methnaol butyrophenone Methnaol valerophenone Methnaol naphthalene Methnaol p-xylene Methnaol Impurity-F Methnaol hexanophenone Acetonitrile benzylalcohol Acetonitrile ibuprofen Acetonitrile benzonitrile Compound 2 ibuprofen p-chlorophenol methylbenzoate anisole propiophenone p-nitrotoluene p-nitrobenzylchloride butyrophenone toluene naphthalene ethylbenzene Impurity-E Impurity-G propylbenzene acetanilide 3-phenylpropanol p-chlorophenol Compound 3 hexanophenone Impurity-F p-xylene bromobenzene p-chlorophenol p-nitrobenzylchloride diethylformamide n-butylbenzene p-nitrobenzylchloride 3-phenylpropanol butyrophenone heptanophenone naphthalene p-chlorophenol p-xylene propiophenone p-chlorophenol Compound 4 heptanophenone p-chlorophenol 3-phenylpropanol Impurity-G propylbenzene benzene propylbenzene p-nitrobenzylchloride acetanilide Impurity-E valerophenone 3-phenylpropanol p-chlorophenol valerophenone valerophenone Impurity-F ethylparaben As a final virtual lab for the semester I would like each of you to determine the best isocratic separation for a set of four analyte compounds

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A B ? D E F G H Last Name First Name Unknown # Fernandes Foster Gilani Gonzales Hackbarth Hamlin Hartenberger Hastings Hauser Jimenez Khoury Lee Lehman Leodones Macario Manfredi Marcos Kevin Legend Shannon Sophia Jayden Mary Joseph Reina Neil Raul Rachel Sofia Lindsey Karla Denise Ashley Brittany Mallory HPLC-25 HPLC-26 HPLC-27 HPLC-28 HPLC-29 HPLC-30 HPLC-31 HPLC-32 HPLC-33 HPLC-34 HPLC-35 HPLC-36 HPLC-37 HPLC-38 HPLC-39 HPLC-40 HPLC-41 Mobile Phase B Compound 1 Methnaol Impurity-C Methnaol benzonitrile Methnaol propylparaben Methnaol methylbenzoate Methnaol anisole Methnaol benzene Methnaol p-nitrotoluene Methnaol butylparaben Methnaol butyrophenone Methnaol valerophenone Methnaol naphthalene Methnaol p-xylene Methnaol Impurity-F Methnaol hexanophenone Acetonitrile benzylalcohol Acetonitrile ibuprofen Acetonitrile benzonitrile Compound 2 ibuprofen p-chlorophenol methylbenzoate anisole propiophenone p-nitrotoluene p-nitrobenzylchloride butyrophenone toluene naphthalene ethylbenzene Impurity-E Impurity-G propylbenzene acetanilide 3-phenylpropanol p-chlorophenol Compound 3 hexanophenone Impurity-F p-xylene bromobenzene p-chlorophenol p-nitrobenzylchloride diethylformamide n-butylbenzene p-nitrobenzylchloride 3-phenylpropanol butyrophenone heptanophenone naphthalene p-chlorophenol p-xylene propiophenone p-chlorophenol Compound 4 heptanophenone p-chlorophenol 3-phenylpropanol Impurity-G propylbenzene benzene propylbenzene p-nitrobenzylchloride acetanilide Impurity-E valerophenone 3-phenylpropanol p-chlorophenol valerophenone valerophenone Impurity-F ethylparaben As a final virtual lab for the semester I would like each of you to determine the best isocratic separation for a set of four analyte compounds. In this table you will find your unknown number 2, your four compounds, and the organic solvent (B) that you are to use. Using the HPLC simulator 2, determine what the best mobile phase composition is for the separation of your compounds with your given solvent. Keep in mind that the best separation is the fastest separation with all the compounds being resolved". For this lab you will need to report three items: 1. The %B for the mobile phase to get your best separation 2. The pair of compounds that elute the closest to each other* 3. The resolution for those two compounds To calculate the resolution you can use the data in the table beneath the chromatograms produced in the simulation. The resolution equation is R = (tr2 - tra)/(0.5*(w? + W2) and a minimum resolution of 1.5 is needed to have properly resolved compounds. tri is the first (fastest) eluting of the two compounds, and w is the width of the base of the respective peak. The table below the chromatogram provides you with the tr values (in minutes), but you need to calculate w, fortunately that is easy to do, simply multiply the o value by 4 (w = 40). When calculating R you need to have the units of time for tr and w the same. Since o is in seconds, multiply to by 60 to convert it to seconds as well. Report you results to this Google form 2. The lab results need to be submitted by the end of the day on May 14th, though I encourage you to get this done before the final exams. *Note that for some of you it may not be possible to get a perfect separation, you may have a pair of compounds that co-elute under all conditions. If that is the case find the best possible resolution between that pair of compounds and report that resolution.