Lab 8- Spectrophotometry of Riboflavin Lambda max: _____________ Table 1: Standard curve data for riboflavin Tube # 1 2 3 4 5 6 53

Lab 8- Spectrophotometry of Riboflavin Lambda max: _____________ Table 1: Standard curve data for riboflavin Tube # 1 2 3 4 5 6 53.1µM Riboflavin (mL) 0.00 2.00 4.00 6.00 8.00 10.00 Distilled Water (mL) 10.00 8.00 6.00 4.00 2.00 0.00 Concentration (µM) Absorbance Equation of the line of best fit from standard curve: y = __________x + ____________ Table 2: Unknown sample calculations Sample y (absorbance) 1 2 = = = m x (concentration) + b + + m and b do not change, use the values from the line of best fit. y is from the absorbance measurement of the unknown sample. Solve for x (concentration)! Table 3: Extinction coefficient Experimental value of extinction coefficient Theoretical value of extinction coefficient Percent error NOTE: be careful of units here! 12.2 x 103 M-1cm-1