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Homework answers / question archive / You have isolated several DNA sequences from a variety of mouse tissues
You have isolated several DNA sequences from a variety of mouse tissues. You have labeled each one of them with a radioisotope and will use them as probes on blots of several DNA and RNA samples.
Below are a list of all the probes you generated (probes A through E) and a list of all the DNA and RNA samples that you will analyze (Samples 1 through 3)
Beside each sample, write the letters corresponding to all the probes that will bind to a complementary sequence in that sample. These responses are graded all or nothing!
List of probes
Probe A: promoter sequence of a gene that is only expressed in the nervous system
Probe B: promoter sequence of one of the genes encoding a ubiquitously expressed histone protein
Probe C: coding sequence of a gene that is only expressed in the nervous system
Probe D: coding sequence of one of the genes encoding a ubiquitously expressed histone protein
Probe E: intron of a gene that is only expressed in the nervous system
List of samples
Sample 1: Southern blot of genomic DNA extracted from adult mouse cardiac muscle system cells
Sample 2: Northern blot of mRNA extracted from adult mouse nervous system cells
Sample 3: Northern blot of mRNA extracted from adult mouse cardiac muscle cells
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