- HIV infection of a susceptible cell is initiated when the gp120 envelope protein binds the _______ antigen
CD4
- HIV is a type of
retrovirus (rna virus)
- HIV possesses a ________ genome
a. ssDNA
b. dsDNA
c. ssRNA
d. dsRNA
e. None of the above are correct.
C
- A type of column chromatography that uses a calcium-phosphate gel. It binds to double-stranded nucleic acid molecules more tenaciously than to single-stranded molecules, double-stranded DNA (dsDNA) can be effectively separated from single stranded DNA (ssDNA), RNA, and protein contaminants by eluting the column with increasing concentrations of phosphate buffer.
Hydroxyapatite
- used to located specific nucleic acid sequences,specifially for DNA: techniques used to identify specific genes, utilizes restriction enzymes, chain terminating
Southern blotting
- The genetic variations observed in DNA fingerprinting are referred to as ______.
RFLPs
- The extraction and purification of bacterial DNA involves the use of which of the following:
Centrifugation (salting out), chromatography (gel-filtration, ion-exchange, affinity), electrophoresis, and extraction
- Palindromes
a. form cruciforms.
b. contain inverted repeats.
c. are sequences that provide the same information whether they are read forwards or backwards.
d. play a role in the function of restriction enzymes.
e. All of the above are correct.
E
- The denaturation of DNA is
A) promoted by heat, low salt concentration, and extremes in pH.
B) reversible.
C) monitored by measuring the absorbance at 300 nm.
D) Both A and B are correct.
E) All of the above are correct.
D
- The chain terminating method for sequencing DNA was developed by
Frederick Sanger