HIV infection of a susceptible cell is initiated when the gp120 envelope protein binds the _______ antigen HIV is a type of HIV possesses a ________ genome a

1. HIV infection of a susceptible cell is initiated when the gp120 envelope protein binds the _______ antigen

CD4

2. HIV is a type of

retrovirus (rna virus)

3. HIV possesses a ________ genome
   a. ssDNA
   b. dsDNA
   c. ssRNA
   d. dsRNA
   e. None of the above are correct.

C

4. A type of column chromatography that uses a calcium-phosphate gel. It binds to double-stranded nucleic acid molecules more tenaciously than to single-stranded molecules, double-stranded DNA (dsDNA) can be effectively separated from single stranded DNA (ssDNA), RNA, and protein contaminants by eluting the column with increasing concentrations of phosphate buffer.

Hydroxyapatite

5. used to located specific nucleic acid sequences,specifially for DNA: techniques used to identify specific genes, utilizes restriction enzymes, chain terminating

Southern blotting

6. The genetic variations observed in DNA fingerprinting are referred to as ______.

RFLPs

7. The extraction and purification of bacterial DNA involves the use of which of the following:

Centrifugation (salting out), chromatography (gel-filtration, ion-exchange, affinity), electrophoresis, and extraction

8. Palindromes
   a. form cruciforms.
   b. contain inverted repeats.
   c. are sequences that provide the same information whether they are read forwards or backwards.
   d. play a role in the function of restriction enzymes.
   e. All of the above are correct.

E

9. The denaturation of DNA is
   A) promoted by heat, low salt concentration, and extremes in pH.
   B) reversible.
   C) monitored by measuring the absorbance at 300 nm.
   D) Both A and B are correct.
   E) All of the above are correct.

D

10. The chain terminating method for sequencing DNA was developed by

Frederick Sanger