Gateway Technical College BIO 806-177 Lecture 8 : Enzyme Kinetics 1)Catalysts primarily work by: Providing additional kinetic energy for a substrate to overcome free energy barriers Increasing the free energy of the substrate Stabilizing the transition state of a reaction Decreasing the free energy of the product Given the reaction S ? P which is catalyzed by an enzyme E, which of the following is TRUE? E increases the rate of the reverse reaction P ? S E reduces change in G+ to 0 The reaction rate is only increased if change in G+ cat > change in G0 non The change in reaction rate depends on change in G0 In a Lineweaver-Burk plot of a reaction without inhibition, if the x-intercept is 0

Gateway Technical College

BIO 806-177

Lecture 8 : Enzyme Kinetics

1)Catalysts primarily work by:

1. 1. Providing additional kinetic energy for a substrate to overcome free energy barriers
   2. Increasing the free energy of the substrate
   3. Stabilizing the transition state of a reaction
   4. Decreasing the free energy of the product
2. Given the reaction S ? P which is catalyzed by an enzyme E, which of the following is TRUE?
   1. E increases the rate of the reverse reaction P ? S
   2. E reduces change in G+ to 0
   3. The reaction rate is only increased if change in G+ cat > change in G0 non
   4. The change in reaction rate depends on change in G0
3. In a Lineweaver-Burk plot of a reaction without inhibition, if the x-intercept is 0.02L/umol and the y intercept is 0.01L min/umol, what is Km?
   1. 2 umol/L
   2. 50 umol/L
   3. 100 umol/L
   4. 0.5 umol/L
4. In a Lineweaver Burk plot of a reaction without inhibition, if the x intercept is 0.02 L/umol and the y intercept is 0.01 L*min/umol, what is Vmax?
   1. 0.5 umol/(L*min)
   2. 2 umol/(L*min)
   3. 50 umol/(L*min)
   4. 100 umol/(L*min)
5. In the case of competitive inhibition, which of the following statements about the Lineweaver-Burk plot is TRUE?
   1. The slope does not change as inhibitor is added
   2. The intersection point as inhibitor is added is given by [-1/a, 1/a]
   3. The y intercept does not change as inhibitor is added
   4. The x intercept does not change as inhibitor is added
6. In case of uncompetitive inhibition, which of the following statements about the Lineweaver-Burk plot is TRUE?
   1. The slope does not change as inhibitor is added
   2. The x intercept does not change as inhibitor is added
   3. The y intercept does not change as inhibitor is added
   4. The intersection point as inhibitor is added is given by [-1/a’, 1/a’]
7. If a Lineweaver-Burk plot of a reaction is not linear, which of the following is TRUE?
   1. The reaction is preformed with a competitive inhibitor
   2. The reaction is performed with a noncompetitive inhibitor
   3. The reaction is performed with a mixed inhibitor
   4. The reaction does not obey Michaelis-Menton kinetics