Pima County Community College BIO 202IN PhysioEx Lab Report Exercise 12: Serological Testing Activity 2: Comparing Samples with Ouchterlony Double Di?usion Date: 31 October 2019 Pre-lab Quiz Results   1)When two antigens are identical their precipitin lines form Antigen and antibody move toward each other because of If two antigens form a spur, they   The Ouchterlony test relies on the formation of a(n) Experiment Results Predict Question 1 Predict Question: How do you think human serum albumin and bovine serum albumin will compare?   Stop & Think Question 1 Which of the following best describes the two solutions you just added to well 1?     Experiment Data   Wells Identity 2 and 5 Partial 2 and 3 Identity 3 and 4 Partial 4 and 5 Identity             Post-lab Quiz Results   There is a spur between wells 2 and 5 and between wells 3 and 4 because the What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)?   Which of the following best describes this test?     The antigen-antibody precipitate forms where the ratio of antigen and antibody is Review Sheet Results Describe how you were able to determine what antigen is in the unknown well

Pima County Community College

BIO 202IN

PhysioEx Lab Report

Exercise 12: Serological Testing

Activity 2: Comparing Samples with Ouchterlony Double Di?usion

Date: 31 October 2019

Pre-lab Quiz Results

 

1)When two antigens are identical their precipitin lines form

2. Antigen and antibody move toward each other because of
3. If two antigens form a spur, they

 

4. The Ouchterlony test relies on the formation of a(n)

Experiment Results

Predict Question

1 Predict Question: How do you think human serum albumin and bovine serum albumin will compare?

 

Stop & Think Question

1 Which of the following best describes the two solutions you just added to well 1?

 

 

Experiment Data

 

Wells	Identity
2 and 5	Partial
2 and 3	Identity
3 and 4	Partial
4 and 5	Identity

 

 

 

 

Post-lab Quiz Results

 

1. There is a spur between wells 2 and 5 and between wells 3 and 4 because the
2. What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)?

 

3. Which of the following best describes this test?

 

 

4. The antigen-antibody precipitate forms where the ratio of antigen and antibody is

Review Sheet Results

1. Describe how you were able to determine what antigen is in the unknown well.
2. Why does the precipitin line form?
3. Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction?