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University of Texas, El Paso BIOLOGY 2311 Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results 1)When two antigens are identical their precipitin lines form   Antigen and antibody move toward each other because of   If two antigens form a spur, they   The Ouchterlony test relies on the formation of a(n)     Experiment Results Predict Question: Predict Question1: How do you think human serum albumin and bovine serum albumin will compare?     Stop & Think Questions: 2

Biology May 04, 2021

University of Texas, El Paso

BIOLOGY 2311

Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results

1)When two antigens are identical their precipitin lines form

 

  1. Antigen and antibody move toward each other because of

 

  1. If two antigens form a spur, they

 

  1. The Ouchterlony test relies on the formation of a(n)

 

 

Experiment Results Predict Question:

Predict Question1: How do you think human serum albumin and bovine serum albumin will compare?  

 

Stop & Think Questions:

2. Which of the following best describes the two solutions you just added to well 1?

 

Experiment Data:

 

Wells

Identity

2 and 5

Partial

2 and 3

Identity

3 and 4

Partial

4 and 5

Identity

 

 

 
 
 

 

 

Post-lab Quiz Results

 

  1. There is a spur between wells 2 and 5 and between wells 3 and 4 because the

 

  1. What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)?

 

  1. Which of the following best describes this test?

 

  1. The antigen-antibody precipitate forms where the ratio of antigen and antibody is

 

 

Review Sheet Results

  1. Describe how you were able to determine what antigen is in the unknown well.

 

  1. Why does the precipitin line form?  

 

  1. Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction?

 

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