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University of Texas, El Paso BIOLOGY 2311 Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results 1)When two antigens are identical their precipitin lines form Antigen and antibody move toward each other because of If two antigens form a spur, they The Ouchterlony test relies on the formation of a(n) Experiment Results Predict Question: Predict Question1: How do you think human serum albumin and bovine serum albumin will compare? Stop & Think Questions: 2
University of Texas, El Paso
BIOLOGY 2311
Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results
1)When two antigens are identical their precipitin lines form
- Antigen and antibody move toward each other because of
- If two antigens form a spur, they
- The Ouchterlony test relies on the formation of a(n)
Experiment Results Predict Question:
Predict Question1: How do you think human serum albumin and bovine serum albumin will compare?
Stop & Think Questions:
2. Which of the following best describes the two solutions you just added to well 1?
Experiment Data:
|
Wells |
Identity |
|
2 and 5 |
Partial |
|
2 and 3 |
Identity |
|
3 and 4 |
Partial |
|
4 and 5 |
Identity |
|
|
Post-lab Quiz Results
- There is a spur between wells 2 and 5 and between wells 3 and 4 because the
- What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)?
- Which of the following best describes this test?
- The antigen-antibody precipitate forms where the ratio of antigen and antibody is
Review Sheet Results
- Describe how you were able to determine what antigen is in the unknown well.
- Why does the precipitin line form?
- Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction?
Expert Solution
PFA
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