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Irvine Valley CollegeBIO BIO 11 Activity 4 Western Blotting Technique 1)Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV

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BIO 11

Activity 4 Western Blotting Technique

1)Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV. 

2. Explain the procedure for a patient with an indeterminate HIV Western blot result.

3. Briefly describe how the nitrocellulose strips were prepared before the patient samples were added to them.

4. Describe the importance of the washing steps in the procedure.