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Ramapo College Of New Jersey BIOL 213-01 Exercise 8: Chemical and Physical Processes of Digestion: Activity 2: Exploring Amylase Substrate Specificity Lab Report Pre-lab Quiz Results 1)Which of the following is true of enzymes and substrates? Which of the following is/are reducing sugars? Cellulose and starch are both Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide bond
Ramapo College Of New Jersey
BIOL 213-01
Exercise 8: Chemical and Physical Processes of Digestion: Activity 2: Exploring Amylase Substrate Specificity Lab Report Pre-lab Quiz Results
1)Which of the following is true of enzymes and substrates?
- Which of the following is/are reducing sugars?
- Cellulose and starch are both
- Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide bond. Which of the following enzymes do you think would be specific for a peptide bond?
- The Benedict's assay tests for the presence of
Experiment Results Predict Question:
Predict Question1: Do you think test tube 3 will show a positive Benedict's test?
2. Which tube is included to detect contaminating amylase in the buffer or in cellulose?
3. What is the usual substrate for the pancreatic enzyme peptidase?
Experiment Data:
|
Tube No. |
Reagent 1 |
Reagent 2 |
Reagent 3 |
Time |
Temp. |
IKI |
Benedict's |
|
1 |
Amylase |
Starch |
pH 7.0 Buffer |
60 |
37 |
- |
++ |
|
2 |
Amylase |
Glucose |
pH 7.0 Buffer |
60 |
37 |
- |
++ |
|
3 |
Amylase |
Cellulose |
pH 7.0 Buffer |
60 |
37 |
+ |
- |
|
4 |
Cellulose |
pH 7.0 Buffer |
Deionized Water |
60 |
37 |
+ |
- |
|
5 |
Peptidase |
Starch |
pH 7.0 Buffer |
60 |
37 |
+ |
- |
|
6 |
Bacteria |
Cellulose |
pH 7.0 Buffer |
60 |
37 |
- |
++ |
Post-lab Quiz Results
- The substrate(s) for amylase is/are
- The results of tube 5 demonstrated that
- Why was the cellulose in tube 6 hydrolyzed to glucose?
- Can you detect the presence of contaminating amylase from your experiments?
Review Sheet Results
- Describe why the results in tube 1 and tube 2 are the same.
- Describe the result in tube 3. How well did the results compare with your prediction?
- Describe the usual substrate for peptidase.
- Explain how bacteria can aid in digestion.
Expert Solution
PFA
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