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Florida International University PCB 3063 15
Florida International University
PCB 3063
15.1Prokaryotic Transcription
1)Describe a prokaryote.
- How is a bacterial chromosome constructed and how is it different from eukaryotic chromosomes?
- What are plasmids?
- When are plasmids transferred and they often carry what traits?
- Transcription requires what to happen to the DNA?
- What is this called?
- What is the template strand?
- Describe the mRNA product?
- What is different in mRNA?
- What is the +1 or initiation site?
- Nucleotides preceding the initiation site are given what types of numbers, are denoted how, and are called what?
- Because prokaryotes do not have membrane-enclosed nuclei, the processes of transcription, translation, and mRNA degradation can occur how?
- Prokaryotes use what to transcribe all of their genes?
- Describe the polymerase holoenzyme.
- What is a promoter?
- In most cases, where to the promoters exist?
- Why is the specific sequence of the promoter so important?
- Where are the two promoter consensus sequences found?
- What is the role of each of these consensus sequences?
- The transcription elongation phase begins with what?
- This allows what to occur?
- During elongation, the prokaryotic RNA polymerase does what?
- Once a gene is transcribed, what has to happen to the prokaryotic polymerase?
- What are the two types of termination signals?
- By the time termination occurs, the prokaryotic transcript would have already been used how?
- All of these processes occur in what direction?
- In prokaryotes, mRNA synthesis is initiated where which is made up of what?
- The prokaryotic polymerase consists of what?
- Elongation synthesizes mRNA in what direction and at what rate?
- Termination liberates the mRNA and occurs how?
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