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1) Explain the purpose of using ethanol as the first extracting solvent

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1) Explain the purpose of using ethanol as the first extracting solvent.  
2.  What is the purpose of adding Na2SO4 to the hexane extract after the liquid – liquid extraction?3.  Explain why the solvent bath is prepared ahead of time and allowed to sit for an hour with the lid on.
4.  Why is it necessary to measure the depth of the solvent before drawing the pencil line on your TLC plate?
5.  Explain the effect of breaking the surface when drawing the pencil line.
6.  Explain the effect of not drying the spots before placing the plate in the developing solvent.7.  Why is it necessary to mark the solvent front immediately.
8.discuss the relative polarities for each pigment (chlorophyll a, chlorophyll b and xanthophyll) with regard to the structure.
9. List three sources of errors with type of errors during experiment and discuss how they effect the results.