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The advantage of treating separate samples of a protein with two or more enzymes when sequencing a protein is that the products are a
The advantage of treating separate samples of a protein with two or more enzymes when sequencing a protein is that the products are
a. more homogeneous
b. sequenceable with out further chromatography
c. fragments with the same N- and C-terminal amino acids
d. fragments with sequence overlaps
e. all are true
Expert Solution
When performing enzymatic digestion of proteins, it is important to use more than one type of protease so that we generate fragments with sequence overlaps. Then, once we sequence each fragment, the overlaps allow us to piece the entire protein sequence together. Without overlaps, we wouldn't know which fragment is adjacent to which, thus never being able to determine the complete sequence in order.
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