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Using Excel and the data set below, generate 2 graphs: Dilution Factor vs

Biology May 03, 2023

Using Excel and the data set below, generate 2 graphs:

  • Dilution Factor vs. Absorbance
  • Cell concentration vs Absorbance

OD @ 600nm

  1. 1/2 dilution - 0.475
  2. 1/4 dilution - 0.347
  3. 1/8 dilution - 0.285
  4. 1/16 dilution - 0.142

To calculate the cell concentration from the OD you need to use the ratio: bacterial cell cultures OD600 of 1.0 = 8 x 10^8cells/ml.

When appropriate include trendlines and equations of the line on each graph. 

Compile these graphs in a Word document. Include a short discussion about the data. 

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