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Using Excel and the data set below, generate 2 graphs: Dilution Factor vs
Using Excel and the data set below, generate 2 graphs:
- Dilution Factor vs. Absorbance
- Cell concentration vs Absorbance
OD @ 600nm
- 1/2 dilution - 0.475
- 1/4 dilution - 0.347
- 1/8 dilution - 0.285
- 1/16 dilution - 0.142
To calculate the cell concentration from the OD you need to use the ratio: bacterial cell cultures OD600 of 1.0 = 8 x 10^8cells/ml.
When appropriate include trendlines and equations of the line on each graph.
Compile these graphs in a Word document. Include a short discussion about the data.
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