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They restrict the DNA, so that it cannot do transcription, translation and make proteins

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They restrict the DNA, so that it cannot do transcription, translation and make proteins. They restrict the DNA, reducing it's amount by half in whatever organism they are used in. They cut DNA at very speci c places. They cut DNA, but anywhere they touch so they are very inaccurate. 2 What is a recognition site? 1 The place at which a GMO can be recognized as not "natural". The place at which a restriction enzyme cuts. The place where a plasmid crosses through the cell membrane. The place where https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 1/7 4/29/2021 Formative 3 2 You have a sample of DNA and also the EcoR1 Enzyme which cuts as shown below. On the sample of DNA, draw in lines at each place where this enzyme would cut. https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 2/7 4/29/2021 Formative 4 3 You have a sample of DNA and also the choice of two enzymes: EcoRV and Taq1. Eco RV cuts as shown below. Taq1 cuts as shown below. If the gene of interest that you want to cut out is highlighted in GREEN. Choose one restriction enzyme from the two choices above and make your cuts. https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 3/7 4/29/2021 Formative 5 1 What kind of ends did the enzyme in question 4 leave on the cut DNA? sticky blunt circular oxidized 6 What does GMO stand for? 1 Genetically Modi ed Organism Gene Manipulation Operation Genetic Maintenance Operon Gene Multiplication Option 7 1 In your own words, explain how GMOs are made. (in general) _____________________________________________________________________ _____________________________________________________________________ ______________________________________________________________ 8 2 You picked a food GMO to research. What was your GMO and explain what modi cation was made to it. _____________________________________________________________________ _____________________________________________________________________ ______________________________________________________________ The rest of this quiz will be about the pBLU lab. https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 4/7 4/29/2021 Formative 9 1 In the lab, the test tubes were on ice, then submerged into a hot water bath, then placed back on ice. This process is known as heat shock. What does a heat shock do? It turns on the plasmid. It allows for the plasmid to enter through the cell membrane. It gives the plasmid a blue color. It causes millions of E.Coli colonies to form in each test tube. 10 There were 6 agar plates in this lab as shown below. 2 Notice that each plate has been numbered with a red number. From the Plates 1-3, which one(s) should have bacteria growing on them? Explain why bacteria can grow here. _____________________________________________________________________ _____________________________________________________________________ ______________________________________________________________ https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 5/7 4/29/2021 Formative 11 There were 6 agar plates in this lab as shown below. 3 Notice that each plate has been numbered with a red number. From the Plates 4-6, which one(s) should have bacteria growing on them? Explain why bacteria can grow here. _____________________________________________________________________ _____________________________________________________________________ ______________________________________________________________ 12 There were 6 agar plates in this lab as shown below. 2 Notice that each plate has been numbered with a red number. From the Plates 1-6, which one(s) should have blue bacteria growing on them? Explain why bacteria the bacteria are blue here. _____________________________________________________________________ _____________________________________________________________________ ______________________________________________________________ https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 6/7 4/29/2021 Formative 13 2 EXTRA CREDIT: You not only researched a GMO, but you learned about several of the GMOs that several of your classmates researched and you took notes on one of them. For your extra credit, who's project did you take notes on? What is the GMO and how was it modi ed? What is one ris and one bene t of their GMO? (This is extra credit, so if you can't answer it/don't want to, then just leave it blank and it won't count against you.) _____________________________________________________________________ _____________________________________________________________________ ______________________________________________________________ https://goformative.com/formatives/6054e58373ab6e81ce0697a3/print 7/7 DNA Fingerprinting Lab A crime has been committed! There was DNA left at the crime scene. Detectives have narrowed it down to 5 suspects. It is up to you to determine which of the suspects has committed the crime, based off of the DNA forensic evidence. 2 Let’s revisit the 5 steps involved in DNA Fingerprinting 1. Extract the DNA from the cells. 2. Make copies of the DNA. 3. Use a restriction enzyme to cut the minisattelites out. 4. Run the minisatallites in a gel. 5. Analyze the gel results. 3 For the sake of this lab, we’re going to say that the DNA has already been extracted from the cells. Also, copies of the DNA has also been made. So we’ll be starting this lab at step 3 with the restriction enzyme. 4 On the next slide, there’s a video clip. Answer the following questions as you watch the video clip. 1. What does the DNA in the vials look like? 2. Did the DNA samples look different from each other? 3. Write down which DNA sample is placed into which color tube. 4. Other than DNA, what was added to each tube? 5. What was the micropipette set to? 6. How long did the tubes have to incubate for? 5 6 On the next slide, there’s one more video clip. Answer the following questions as you watch the video clip. 1. What was added to each tube after the incubation? Why was it added? 2. What was the micropipette set to? 3. Write down which lane each DNA sample ended up in. 4. Knowing what you know about DNA, which pole do you think it will travel towards: the positive or the negative? 5. Which fragments (small or large) will end up closer to the wells and which ones will end up further away? 6. How long does the gel run for? 7 8 Now What? Did you answer those questions? (either on a piece of paper or a google doc) Good! Hold onto it...you’ll end up turning it in, but later after we have our results. Speaking of results...you will be tuning in Thursday “live” on Zoom and you’ll get to see the results during class along with everyone else. 9 Now that you’ve seen the results of the lab, answer the following questions right below your video questions. Results!! 1. What biological molecule was actually in each band on the gel? 2. Why is there more than one band for each of the samples? 3. What caused the DNA to become fragmented? 4. Which sample had the smallest DNA fragment? Explain. 5. What would the gel have looked like if the DNA were not fragmented? 6. What determines where a restriction enzyme will cut? 7. Based off of your gel, which suspect left their DNA at the crime scene? Explain. 8. Based what you know about CODIS sites, what are the odds that someone else other than your suspect left their DNA at the crime scene? 10 Picture of the gel results 11 All done? Take a picture of your questions from the 2 lab videos and the results and upload them to PSL. Athletic Training Reviewing Set 1 Muscle Assignment – FIB 2 Directional Video A Using the Language of Movement Directional Term Sheet show a physical example of what is meant by each one. IF in-person you will have a buddy to do it with. IF remaining a Remote-Learner, you will have to do all of them yourself unless you can coordinate with another online person how to work together to create 1 video in which 1 of you states the word and the other person does the action. Quest on Directional Terms Anatomical Positions & Terms Pertaining to Direction &/Or Function 1. All body positions are given relative to: Observer 2. Sideways Caudal Posterior Exterior Caudal Coronal Anterior Distal If a body part is said to be “behind” another we say it is? Occluded 6. Backward What term is used to describe a body part nearer to the front of the body? Abdominal 5. Anatomical Position What term is used to describe a body part nearer to the head? Superior 4. Midline of body In the anatomical position, the palms of the hands face --Forward 3. Visceral Distal Posterior Supportive Which plane divides the body into upper and lower halves? Frontal Coronal Transverse Sagittal 7. Which plane would divide the body into anterior and posterior portions? Frontal 8. Coronal Sagittal Which plane divides the body into left and right halves? Frontal 9. Transverse Coronal Transverse Sagittal Turning one’s sole of their foot outward? Dorsi flexion Inversion Plantar Flexion Proximal Eversion 10. Bending one’s arm up into a bicep curl position would be what type of motion? Eversion Proximal Extension Flexion Inversion 11. The movement away from the axis or trunk is known as? Adduction Medial Abduction Lateral Superficial 12. Moving the top of the foot toward the anterior tibia bone is? Dorsiflexion Plantar flexion Inversion Eversion 13. Occurs when a ball & socket joint encompasses several directions with one movement? Adduction Circumduction Dorsiflexion Abduction 14. Straightening or moving bones apart is? Eversion Flexion Proximal Extension 15. The movement toward the axis or trunk is known as? Adduction Lateral Medial Abduction 16. Means closer to the trunk? Dorsi flexion Distal Proximal Eversion Proximal Eversion 17. Means away from an attachment? Dorsi flexion Distal 18. Toward the midline of the body is known as? Adduction Medial Abduction Lateral 19. Occurs when the palms are turned down? Protraction Supination Inversion Retraction Eversion Pronation 20. When a body part is located away from the midline it is said to be? Adduction Medial Abduction 21. Occurs when the sole of the foot is turned inward? Lateral Dorsi flexion Inversion Plantar Flexion Proximal Eversion 22. Occurs when the palms are turned up? Protraction Supination Inversion Retraction Eversion Pronation 23. Means toward the feet? Protraction Retraction Cranial Inversion Supination Pronation Caudal Pronation Caudal 24. Means toward the head? Protraction Retraction Cranial Inversion Supination 25. This means toward the surface of the body? Dorsal Superficial Ventral Deep Cranial Caudal Cranial Caudal Cranial Caudal 26. This means towards the front of the body? Dorsal Superficial Ventral Deep 27. This means toward the back to the body? Dorsal Superficial Ventral Deep 28. This means inside the body, away from the surface? Dorsal Superficial Ventral Deep Cranial Caudal Chapter 7 Understanding Athletic Training Chapter 7 Understanding Athletic Injury: Terminology & Classifications Intro to Athletic Training: Student Primer Book 1. The proper function of any anatomical area is dictated by what? (1 pt) 2. Give an example of how the function is dictated by the answer in # 1? (1 pt 3. How is it recommended one develop a professional vocabulary in medical terminology? (1 pt) 4. What is one of the most common documentation techniques used in the medical field? Define and explain each component. (9 pts 5. Explain why it is so important that athletic training students use appropriate language when referring to a medical injury? (1 pt) 6. An injury that has a slow onset and long duration is called _________________ injury. 7. A collection of signs and symptoms is known as a ___________________. 8. Explain the difference between a sign and a symptom. (2 pts) 9. The complete disarticulation of a joint is known as a ___________________________. 10. An injury to muscular tissue or the muscle tendon complex is known as a _________________. 11. A recent injury with a rapid onset is known as what type of injury?_____________________ 12. A collection of fluid contained within a joint space or joint capsule is known as ______________ 13. The suffix –osis means what? __________________________________________ 14. Swelling as a result of the collection of fluid in connective tissue is known as?________________ 15. A partial or incomplete disarticulation of a joint followed by an immediate and spontaneous relocation is known as a ________________________. 16. An injury to a joint, most often involves injury to the ligaments and to the joint capsule is a ____________. 17. The suffix –algia refers to what?____________________________________________ 18. The suffix –itis refers to what?______________________________________________ 19. The discoloration that often accompanies # 14 is referred to as _______________________ 20. What type of muscle contraction occurs when a muscle shortens (also known as a positive contraction) ? ________________ 21. What type of muscle contraction occurs when a muscle lengthens against resistance (also known as a negative contraction) ? ________________ 22. Any discussion of movement and body position should begin in terms of what? ____________________ 23. What are the 3 cardinal planes and then state what type of movement takes place within each. (6 pts) 24. What are the 5 cardinal signs of inflammation AND what would be the cause of each. (10 pts) 25. Give an example of an ACUTE injury? (1 pt) 26. Give an example of a CHRONIC injury? (1 pt) 27. How can an athletic trainer determine what stage the athlete is in after an injury based off of the 5 cardinal signs of inflammation? (2 pts) 28. What does MOI stand for and why is it so important? (2 pts 29. When compression, shear and tension are mechanical forces that contribute to injury. Define each: (3 pts) a. Compression: ______________________________________ b. Shear:____________________________________________ c. Tension:__________________________________________ 30. What is the importance of time-loss injury data in athletic training? (1 pt) 31. Define injury epidemiology? (1 pt) 32. Define a catastrophic injury? (1pt) 33. What is the difference between a direct and indirect fatality? (2 pts) 34. What sport is associated with the greatest number of catastrophic injuries? Acronyms, Abbreviations and Symbols Worksheet Create 10 of your own Medical Documentation statements Directions: Create 10 of your own Medical Documentation statements and under each one bullet the statement in actual words! Each one must be vastly different than the other using different documentation abbreviations, symbols, and acronyms (4-5 in each one). Use appropriate terminology if identifying bones and muscles or directional locations in statements. Can not be spinoffs of ones used on the previous sheet. 1. Bone Palpation Practice Videos Your task is to practice palpating the locations of various footbones on the sheet and knowing the names. You will take a video palpating someone's foot which has the following stipulations: #1. What you are pointing to cannot be blocked by anything. #2 Must state the name of the one you are pointing to and use directional terms to describe it. #3 Can only be 2 minutes long. (you must know your stuff!!) This will be due Thursday, February 25th. See attached sheet for the bones that must be in the video. March 8 Bunion Taping & Open Basket Must submit a video of you doing these 2 tapings. If in-person you will video also using the camera stands... and then show me as I cannot watch everyone at once! MAKE SURE you STATE the date with your voice for each video... Monday, March 8th, and what you are doing.